土壤硝化强度测定方法的改进  

Improved Method of Measuring Soil Nitrification Activities

作　　者：史云峰[1,2] 张丽莉[2] 赵牧秋[1,2] 王刚[1] 

机构地区：[1]琼州学院生物科学与技术学院,海南三亚572022 [2]中国科学院沈阳应用生态研究所,沈阳110016

出　　处：《中国农学通报》2014年第33期225-229,共5页Chinese Agricultural Science Bulletin

基　　金：海南省自然科学基金项目"植物源生化抑制剂筛选及对土壤氮转化调控研究"(312100);国家科技支撑计划项目"稳定性肥料关键技术研究及产业化"(2011BAD11B04);三亚市配套项目"植物源生化抑制剂对土壤氮转化的调控作用研究"(2013PT22)

摘　　要：为了使土壤硝化强度的测定条件更加统一,结果更加准确,本研究针对土壤硝化强度测定时广泛采用的悬液法,研究了其最佳培养条件:反应体系p H 7.5,底物NH4+-N浓度为100 mg/L,培养温度为30℃,培养时间为5 h,风干土样预培养72 h后才可进行硝化强度的测定。还针对NO3--N生成法的不足,探讨了通过NO2--N生成法评价硝化强度的途径:通过向反应体系中添加1 mol/L的氯酸钠0.2 m L,有效抑制NO2-向NO3-的氧化。不同检测方法对硝化强度测定对比的结果发现,NO2--N生成法确是评价土壤硝化强度的最佳方法。In order to unify the measurement conditions of soil nitrification activities and increase the accuracyof results, the optimum culture conditions of suspension method for measuring soil nitrification activities wereimproved： the p H of the reaction system was 7.5, the substrate concentration of NH4＋-N was 100 mg/L, culturetemperature was 30℃, culture time was 5 h, and soil nitrification activities was measured after air-dried soilsample pre-cultured for 72 h. The study also aimed at the deficiency of preparation method of NO3--N, anddiscussed the method of measuring soil nitrification activities by the preparation method of NO2-- N. Themethod was adding 1 mol/L sodium chlorate 0.2 m L to the reaction system for effective inhibition of NO2-toNO3-oxidation. By comparing different methods, the preparation method of NO2--N was the best method ofmeasuring soil nitrification activities.

关 键 词：硝化强度 测定方法 土壤 亚硝态氮 氯酸钠 

分 类 号：X833[环境科学与工程—环境工程]