外周血可溶性CD28浓度与Graves疾病的相关性研究  

Correlation analysis between plasma soluble CD28 level and Graves' disease

作　　者：孙中文[1,2] 陶鸿 孙静[2] 黄静芳 易丽娴 陈蕾[3] 

机构地区：[1]江苏省苏州市检验医学生物技术中心重点实验室 [2]苏州卫生职业技术学院医学技术学院,江苏苏州215009 [3]江苏省苏州市立医院内分泌科,215002

出　　处：《检验医学与临床》2014年第23期3240-3242,共3页Laboratory Medicine and Clinic

基　　金：国家自然科学基金资助项目(31100626);江苏省卫生职业技术教育研究立项资助(J201101)

摘　　要：目的探讨Graves病患者外周血中可溶性CD28(sCD28)分子浓度与三碘甲状腺原氨酸(FT3)、游离甲状腺素(FT4)、敏感促甲状腺激素(sTSH)和促甲状腺激素受体抗体(TRAb)等实验室参数的相关性,以分析外周血sCD28分子水平测定对Graves病的临床辅助诊断价值。方法采用双单抗夹心酶联检测法分析Graves病患者血液中sCD28分子的水平;化学发光免疫法检测血液中甲状腺功能指标FT3、FT4、sTSH的浓度;酶联免疫吸附法(ELISA)测定血液中TRAb的水平。结果 Graves病患者血液中sCD28分子水平[(3.42±0.74)ng/mL]明显高于健康对照组[(0.83±0.29)ng/mL],比较差异有统计学意义(P<0.01);患者体内sCD28分子的表达水平与FT3、FT4和TRAb浓度均呈正相关关系(P<0.01),r分别为0.786、0.746和0.734,但sCD28分子浓度与血清sTSH表达呈显著负相关(P<0.01),r为-0.719;外周血液中sCD28分子与Graves病患者甲状腺的肿大程度(P<0.05)和突眼症(P<0.01)均具有显著的正相关关系。结论 sCD28分子参与了Graves病发生发展的病理过程,与FT3、FT4、sTSH和TRAb均具有较好的相关性,可作为Graves病临床辅助诊断和疗效评估的重要生物学参数。Objective To explore the correlation between plasma soluble CD28（sCD28） level and laboratory parameters ,including free triiodothyronine （FT3 ） ,free thyroxine （FT4 ） ,sensitive thyroid stimulating hormone （sTSH）and thyroid stimulating hormone receptor antibody（TRAb） ,and analyze diagnostic value of plasma sCD28 level for Graves＇disease .Methods The double‐sandwich enzyme‐linked immunosorbent assay was used to detect sCD28 ,the chemiluminescence assay was used to detect serum FT3 ,FT4 and sTSH levels ,and enzyme‐linked immu‐nosorbent assay（ELISA） was used to detect serum TRAb level .Results The level of sCD28 in patients with Graves′disease[ （3 .42 ± 0 .74）ng/mL]were obviously higher than that in healthy subjects [（0 .83 ± 0 .29）ng/L] ,with signif‐icant difference（P〈0 .01） .There were significant positive correlation between the serum sCD28 level and FT3 ,FT4 and TRAb levels（P〈0 .01） ,and the correlation coefficients were 0 .786 ,0 .746 and 0 .734 .The serum sCD28 level was significantly and negatively correlated with sTSH level（P〈0 .01） .And there were significant positive correlation between sCD28 level and the degrees of struma（P〈0 .05） ,exophthalmia（P〈0 .01） .Conclusion sCD28 might be in‐volved in the pathological process of the occurrence and development of Graves＇disease;and FT3 ,FT4 ,sTSH and TRAb ,which were correlated with sCD28 ,could be significant parameters in diagnosis and curative effect evaluation of Graves＇disease .

关 键 词：毒性弥漫性甲状腺肿 可溶性CD28分子 相关性 

分 类 号：R581.1[医药卫生—内分泌]