CPS4显色培养基在分离鉴定泌尿道病原菌中的价值  

The application value of CPS4 chromogenic culture media in isolation and identification of urinary pathogens

作　　者：张银旺[1] 荣媛[1] 

机构地区：[1]武汉大学中南医院检验科,武汉430071

出　　处：《检验医学与临床》2014年第23期3272-3274,共3页Laboratory Medicine and Clinic

摘　　要：目的：评估CPS4显色培养基在分离鉴定泌尿道病原菌的诊断价值。方法收集2013年1～12月武汉大学中南医院临床科室送检的中段尿细菌培养标本1074份，以CPS4显色培养基与传统方法分别进行分离鉴定，并比较鉴定结果。结果传统方法分离出病原菌464株，其中大肠埃希菌229株（占49．4％），其次是肠球菌98株（占21．1％），检出复合菌株29例；用CPS4显色培养基分离出病原菌466株，检出复合菌株33例，与传统方法的分离培养性能基本一致，直接鉴定大肠埃希菌、肠球菌、变形杆菌及克雷伯菌属－肠杆菌属－沙雷菌属－枸橼酸杆菌属群（KESC群）的符合率达97％以上，且直接鉴定的细菌数占泌尿系统病原菌85．3％。结论 CPS4显色培养基能简便、快速地分离鉴定临床常见的泌尿道病原菌，且混合菌感染的分离鉴定更优，有利于泌尿道感染的快速诊断。Objective To evaluate the diagnostic value of CPS4 chromogenic culture media in isolation and i‐dentification of urinary pathogen .Methods 1 074 Urine samples were collected and cultured using CPS4 chromogenic and traditional culture media simultaneously ,and the identification results were compared .Results 464 strains of pathogens including 29 compound stains were identified with traditional culture media .The principal pathogens were identified as Escherichia coli（229 ,49 .4% ）and enterococcus（98 ,21 .1% ） .Meanwhile ,466 strains of pathogens inclu‐ding 33 compound stains were identified with CPS4 chromogenic culture media .By comparison ,the performance of CPS4 chromogenic and traditional culture media was basically identical .The accuracy of CPS4 chromogenic culture media was 97% in identifying Escherichia coli ,Enterococcus ,Proteus and the Klebsiella ,Enterobacter ,Serratia and Citrobacter group（KESC） .85 .3% of urinary pathogens can be directly identified with color change by CPS4 chromo‐genic culture media .Conclusion The common urinary tract pathogens can be identified by CPS4 chromogenic culture media fast and conveniently ,especially for multiple infectious samples ,which could be worthy for rapid diagnosis of urinary infection .

关 键 词：显色培养基 尿培养 细菌鉴定 泌尿道感染 

分 类 号：R691.3[医药卫生—泌尿科学]