血培养主要病原菌分布及耐药性分析  

Distribution and drug resistance of main pathogens isolated from blood culture

作　　者：张庆芳[1] 陆桃红[1] 朱晓丽[1] 田凤喜[1] 陈亚宝[1] 

机构地区：[1]泰州市人民医院检验科,江苏泰州225300

出　　处：《国际检验医学杂志》2014年第24期3338-3340,共3页International Journal of Laboratory Medicine

摘　　要：目的分析医院2012年1月至2013年12月血培养中主要病原菌及其耐药性,为临床选择抗菌药物提供依据。方法采集怀疑有血液感染的患者血标本,在BD BACTEC 9120全自动血培养仪中培养,培养阳性者用VITEK-2COMPACT全自动细菌鉴定仪进行细菌鉴定和药物敏感试验。结果医院2012年1月至2013年12月血培养共分离出细菌969株,革兰阳性菌540株,占55.7%;革兰阴性菌413株,占42.6%;真菌16株,占1.7%,所分离的病原菌中前3位分别为表皮葡萄球菌、大肠埃希菌和金黄色葡萄球菌。表皮葡萄球菌和金黄色葡萄球菌对青霉素高度耐药,对万古霉素和利奈唑胺敏感;大肠埃希菌和肺炎克雷伯菌对亚胺培南和哌拉西林/他唑巴坦高度敏感。结论及时了解血培养结果可以为临床抗菌治疗提供依据,对提高治愈率有重要的临床意义。Objective To investigate the distribution and drug resistance of pathogens isolated from blood culture samples from January 2012 to October 2013 to provide the basis for clinical lection of antibacterial drugs.Methods The blood samples were col-lected from the patients with suspected blood infection and cultured by the BD BACTEC 9120 automatic blood culture instrument. The samples with positive results were performed the bacterial identification and the drug sensitivity test by using the VITEK-2 COMPACT automatic bacterial identification instrument.Results A total of 969 strains of pathogens were isolated from blood cul-ture samples,including 540 strains（55.7%）of Gram-positive bacteria,413 strains（42.6%）of Gram-negative bacteria and 16 strains （1.7%）of fungi.The top 3 isolated pathogenic bacteria were Staphylococcus epidermidis,Escherichia coli and Staphylococcus au-reus.Staphylococcus epidermidis and Staphylococcus aureus were highly resistant to penicillin,sensitive to vancomycin and linezol-id;Escherichia coli and Klebsiella pneumoniae were highly sensitive to imipenem and Piperacillin/tazobactam.Conclusion It is nec-essary to understand the blood culture results timely so as to provide the basis for clinical antibacterial therapy and the improvement of the cure rate.

关 键 词：血培养 病原菌 耐药性 

分 类 号：R446.5[医药卫生—诊断学]