环维黄杨星D对大鼠心力衰竭的治疗作用  

Therapeutic Effects of Cyclovirobuxine D on Rats with Heart Failure

作　　者：黄丹丹[1] 邵靓婧[2] 许立[3] 

机构地区：[1]南京军区南京总医院药品科,南京210029 [2]南京军区南京总医院血液病科,南京210002 [3]南京中医药大学药学院,南京210029

出　　处：《医药导报》2014年第12期1562-1565,共4页Herald of Medicine

摘　　要：目的通过观察环维黄杨星D(CVB-D)对心力衰竭(心衰)大鼠心脏功能和心肌形态、结构的影响,探讨CVB-D对心衰的治疗作用。方法结扎SD大鼠冠脉左前降支复制大鼠心衰模型,随机分为5组,另加假手术组。应用超声心动仪检测心功能指标:左室后壁舒张期厚度(LVPWd)、左室后壁收缩期厚度(LVPWs)、左室收缩末期内径(LVIDs)、左室舒张末期内径(LVIDd)、射血分数(EF)、心率(HR)、心输出量(CO)、左室短轴缩短率(LVSF)、每搏输出量(SV);制作心肌组织切片,苏木精-伊红(HE)染色观察心肌组织学改变,透射电镜观察心肌超微结构的变化。结果与模型对照组比较,CVB-D中、大剂量组EF、LVSF明显增加(P<0.05或P<0.01),CVB-D小、中剂量组可见SV增加(P<0.05),CVB-D小、中、大剂量组显示CO增加(P<0.05),CVB-D大剂量组显示LVIDs减少,LVPWs增加(P<0.05)。光镜下CVB-D各剂量组使受损心肌组织出现不同程度的变化:心肌纤维排列逐渐整齐,横纹逐渐明显,轮廓逐渐清晰。透射电镜下心肌超微结构发生变化:CVB-D使受损心肌纤维断裂减少,线粒体数量增多、肿胀变性减轻。结论 CVB-D对心衰大鼠心脏收缩、泵血功能有明显的改善作用,对心肌肌纤维、线粒体的损伤有缓解作用,推测CVB-D对心衰存在一定的治疗作用。Objective To explore the therapeutic effect of cyclovirobuxine-D （CVB-D） on heart failure by observing the influences of CVB-D on cardiac function,myocardial morpbology and structure in rats with heart failure. Methods The rat model of heart failure was established by ligating left anterior descending coronary artery, and then the rats were randomly divided into 5 groups,plus the sham group. Echocardiography was used to assess cardiac function, including EF, LVSF, SV, HR, CO and so on. Myocardial tissue section was obtained. Change in the myocardial tissue was observed by HE staining. Myocardial ultra- structure was observed by using transmission electron microscope. Results As compared with the model group, EF and LVSF were significantly increased in medium and high dose CVB-D groups （ P〈0.05 or P〈0.01 ） , SV was increased in low and medium dose CVB-D groups （P〈 0. 05 ）, CO was increased in low, medium and high dose CVB-D groups （P〈 0.05 ）, LVIDs was decreased and LVPWs was increased in high dose CVB-D group （P〈0.05）. Under light microscope, changes of myocardial structures were observed in different CVB-D dose groups： myocardial fiber gradually became tidy, stripes became clear, and contour became clear. The changes of myocardial ultra-structure were observed under transmission electron microscope： the myocardial fiber breakage was reduced and the number of mitochondria was increased, swelling degeneration was alleviated. Conclusion CVB-D significantly improved cardiac contraction and blood pumping function of rats with heart failure, exerted mitigative effect on damage of cardiac muscle fiber and mitochondria. It suggests that CVB-D may be effective in the treatment of heart failure.

关 键 词：环维黄杨星D 心力衰竭 心功能 

分 类 号：R285.5[医药卫生—中药学] R541[医药卫生—中医学]