rhGM-CSF及纳米银对深Ⅱ度烫伤创面愈合过程血管化的影响  

Effect of Recombinant Human Granulocyte / macrophage Colony-stimulating Factor and Nano-silver on Neovascularization in Healing Process of Skin with Deep Ⅱ Degree Burn

作　　者：杨景哲[1] 温海玲[1] 耿琪瑛[1] 陈凤平[1] 冯欣姝[1] 

机构地区：[1]承德医学院附属医院烧伤整形科,承德067000

出　　处：《医药导报》2014年第12期1570-1574,共5页Herald of Medicine

基　　金：河北省2013年医学科学研究重点课题计划指令性课题(20130024);承德市科学技术研究与发展计划项目(20122164)

摘　　要：目的研究外用重组人粒细胞-巨噬细胞集落刺激因子(rh GM-CSF)和纳米银敷料外用,对深Ⅱ度烫伤创面组织愈合及血管化的影响。方法用Wistar大鼠建立深Ⅱ度烫伤模型,分为A、B、C组。A组(n=30):凡士林纱布覆盖,B组(n=30):纳米银敷料覆盖,C组(n=30):rh GM-CSF涂抹创面。伤后第1,4,7,10,14,21天,观察创面病理学改变,按照酶联免疫吸附法测定血清中血管内皮生长因子(VEGF)水平,采用实时-聚合酶链反应(RT-PCR)方法,检测创面愈合过程中缺血坏死因子-1α(HIF-1α)mRNA表达的变化。结果病理学改变:A、B、C组均在第10天出现明显的血管化;创面愈合率:C组>B组>A组,第14天、第21天时,组间差异有统计学意义(P<0.05)。VEGF水平:伤后第1天3组间差异无统计学意义(P>0.05),第4,7,10,14天3组间及第21天A组和C组之间差异有统计学意义(P<0.05)。HIF-1αmRNA相对表达量:A组第14天达峰值,B组、C组逐渐增大,第10天达峰值,随后下降,且C组下降幅度最明显,第1天A组与B组、C组之间差异有统计学意义(P<0.05),第4天,3组间差异无统计学意义(P>0.05),第7天A组与C组之间及第10,14,21天各组间差异均有统计学意义(P<0.05)。结论 rh GM-CSF和纳米银外用,促进深Ⅱ度烫伤创面愈合过程中血管化的形成,并且rh GM-CSF血管化程度优于纳米银。Objective To observe the effect of recombinant human granulocyte / macrophage colony stimulating factor（ rh GM-CSF） and nano-silver as treatment for skin with deep Ⅱ degree burn. Methods Deep Ⅱ degree burn Wistar rat model was established. The rats were randomly divided into three groups,petrolatum treatment group（ group A,n = 30）,nanosilver treatment group（ group B,n = 30）,and rh GM-CSF treatment group（ group C,n = 30）. The pathological changes of wound of the three groups were observed 1,4,7,10,14 and 21 days after the treatment. The concentration of VEGF in serums was measured with ELISA. The levels of HIF-1α mRNA expression were detected by RT-PCR. Results On day 10,neovascularization developed in groups A,B and C. Healing rate of the wound was the highest in group C,lowest in group A,with significant differences among the three groups on day 14 and day 21（ P〈0. 05）. VEGF level peaked on day 14 in group A,and on day 10 in groups B and C. There were significant differences in VEGF level on day 1 between group A and groups B,C,on day 7 between group A and group C,on day 10,14 and 21 among the three groups（ P〈0. 05）,but no significant differences on day 4 among the three groups. Conclusion rh GM-CSF and nano-silver treatment can accelerate neovascularization of wound,and rh GM-CSF is better than nano-silver.

关 键 词：重组人粒细胞-巨噬细胞集落刺激因子 纳米银 烫伤创面 血管内皮生长因子 缺血坏死因子 

分 类 号：R986[医药卫生—药品] R644[医药卫生—药学]