不同样品溶剂对高效液相色谱-蒸发光散射检测器测定黄芪甲苷的影响  

Effect of Different Sample Solvents on Determination of Astragaloside Ⅳ by High Performance Liquid Chromatograph- Evaporative Light Scattering Detector

作　　者：徐静 刘天强 彭衡阳 肖丹[1,2] 

机构地区：[1]通威股份有限公司,成都610041 [2]成都中医药大学,成都611137

出　　处：《医药导报》2014年第12期1624-1627,共4页Herald of Medicine

基　　金：国家国际科技合作专项资助(2010DFA32380)

摘　　要：目的考察不同样品溶剂对高效液相色谱-蒸发光散射检测器(HPLC-ELSD)测定黄芪甲苷的影响。方法以黄芪和感毒清颗粒为样品,以甲醇、90%甲醇、80%甲醇、70%甲醇、32%乙腈、15%乙腈和水为样品溶剂,采用HPLC-ELSD测定黄芪甲苷的含量。结果以90%甲醇溶液为样品溶剂,其系统适用性良好,准确度、精密度、线性关系等方法学验证指标符合要求。结论采用90%甲醇溶液作为样品溶剂,更有利于黄芪及制剂中黄芪甲苷的质量控制。Objective To explore the effect of sample-solvent compositions on the determination of Astragali Ⅳ by high performance liquid chromatograph- evaporative light scattering detector（ HPLC-ELSD）. Methods Radix astragali and ganduqing granules served as samples. Methanol,90% methanol,80% methanol,70% methanol,32% acetonitrile,15% acetonitrile,and water were sample solvents. HPLC-ELSD was used to determine content of astragaloside Ⅳ. Results The results showed that the 90% methanol solution was an appropriate sample solvent with good system suitability,precision,accuracy,and,linearity etc..Conclusion This method benefits the quality control of astragaloside Ⅳ in Radix astragali and agents containing Radix astragali.

关 键 词：黄芪甲苷 样品溶剂 溶剂效应 色谱 高效液相 检测器 蒸发光散射 

分 类 号：R286[医药卫生—中药学] R927.2[医药卫生—中医学]