雄性半滑舌鳎GH基因多态性与激素水平及生长性状相关性分析  

Correlation Between the Polymorphism of GH Gene of Male Half-Smooth Tongue Sole and Their Growth Traits and Hormone Content

作　　者：赵君丽[1] 何峰[1] 温海深[1] 李吉方[1] 司玉凤 任远源 黄政举 赵梅琳[1] 

机构地区：[1]中国海洋大学水产学院海水产养殖教育部重点实验室,山东青岛266003

出　　处：《中国海洋大学学报（自然科学版）》2014年第12期35-40,共6页Periodical of Ocean University of China

基　　金：国家高技术研究发展计划项目(2012AA10A403)资助

摘　　要：以雄性半滑舌鳎(Cynoglossus semilaevis)为研究材料,分析了其GH基因外显子区域的多态性,同时分析了各基因型与其体重、体长、激素水平等的关联性。PCR-SSCP和测序结果表明,外显子4有1个突变位点382bp(c.T382C)为非同义突变(p.Phe106Leu)。单因素方差分析显示,382bp位点与体重、去内脏重和血浆T3水平显著相关(P<0.05)。通过多重比较,CC基因型体重和去内脏重((234.58±8.11)g和(210.23±7.55)g)显著高于TT基因型((201.64±13.04)g和(180.21±12.13)g);CC基因型(3.01±0.07)ng·mL-1的T3水平极显著高于TT基因型(2.12±0.12)ng·mL-1。结果表明,半滑舌鳎中GH基因的突变可以影响其激素水平和生长性状,382bp位点可以用作分子标记,从遗传学角度辅助半滑舌鳎优良品种的分子选育。The correlation between the polymorphism of exons of GH gene of male half-smooth tongue sole （Cynoglossus semilaevis） and their growth traits and hormone level was determined with polyme- rase chain reaction-single strand conformation polymorphism （PCR-SSCP） and by sequencing. One SNP （c. T382C） located in exon 4 at base 382 was found to be non-synonymous （p. Phel06Leu）. One-way ANOVA showed that this point was significantly associated with weight （P〈0.05）, weight without viscera （P〈0. 05） and serum T3 level （P%0.01）. Duncan multiple-range testing showed that CC geno- type was significantly higher in weight and weight without viscera （（234. 58±8. ll）g and （210. 23± 7.55）g） than TT genotype （（201.64±13.04）g and （180. 21±12.13）g）; and CC genotype was signifi- cantly higher in Ta level （3.01±0. 07） ng/mL than TT genotype （2.12±0. 12） ng/mL. These findings indicated that mutation of GH gene may influence the hormone level and growth traits of male half- smooth tongue sole, and base 382 may serve as a selection assistant maker.

关 键 词：半滑舌鳎 GH基因 单核苷酸多态性 激素水平 生长性状 

分 类 号：S917.4[农业科学—水产科学]