阿托伐他汀对脓毒症大鼠肺组织病理及血清TNF-α、HMGB-1的影响  

Effect of atorvastatin on the expression of TNF-α and HMGB-1 in lung tissue of rats with sepsis

作　　者：蒋建红[1] 刘文明[1] 杨汆[1] 俞建峰[1] 孟丽君[1] 蔡雷铭[2] 关乃富[2] 

机构地区：[1]南京医科大学附属常州市第二人民医院重症医学科,213003 [2]南京医科大学附属常州市第二人民医院病理科,213003

出　　处：《疑难病杂志》2014年第12期1283-1285,F0003,共4页Chinese Journal of Difficult and Complicated Cases

摘　　要：目的探讨阿托伐他汀对脓毒症大鼠肺组织病理及血清肿瘤坏死因子(TNF-α)、高迁移率族蛋白1(H4MGB-1)表达的影响。方法将75只健康SD大鼠随机分为假手术组、脓毒症组及干预组各25只。每组在术后12、24、36、48、72 h各取大鼠5只处死并取心脏血及肺组织,常规行HE染色观察肺组织病理变化,ELISA法检测血清TNF-α水平,Western Blot法检测HMGB-1表达水平。结果假手术组大鼠肺组织可见少量炎性细胞;脓毒症组肺组织及肺泡内可见水肿,大量炎性细胞浸润及红细胞;干预组大鼠肺组织炎性细胞较脓毒症组明显减轻,肺泡结构尚完整,病理改变明显减轻。脓毒症组、干预组肺组织W/D较假手术组明显升高(P<0.01),其中干预组在36 h后明显降低,且低于脓毒症组(P<0.05)。各时间点干预组和脓毒症组TNF-α及HMGB-1表达水平均较假手术组明显升高(P<0.05),且干预组表达水平较脓毒症组明显降低(P<0.05),36 h后干预组和脓毒症组TNF-α及HMGB-1表达水平有所降低(P<0.05)。结论阿托伐他汀可改善脓毒症大鼠肺组织病理炎性损伤,降低血清TNF-α及HMGB-1表达水平。Objective To investigate the effect of atorvastatin on pathology and expression of serum tumor necrosis factor（TNF-a） and high mobility group protein 1（HMGB-1） in lung tissue of rats with sepsis.Methods Seventy-five healthy SD rats were randomly divided into sham operation group,sepsis group and intervention group with 25 rats in each group.In each group,after 12,24,36,48,72 h,5 rats from each group were sacrificed and blood of the heart and lung tissue were sampled,lung tissue routine HE staining were perfomred to observe the pathological changes,serum TNF-a level were determined with ELISA method,expression of HMGB-1 were detected by Western Blot method.Results A few inflammatory cells were found in the sham operation group;Lung tissue and alveolar edema,infiltration of inflammatory cells and red blood cells were found in sepsis group;intervention group＇ s inflammatory cells in lung tissue were less than sepsis group,the alveolar structure is complete,the pathological changes were obviously relieved.Sepsis group and intervention group＇ s lung tissue＇s W/D were higher than those in the sham operation group（P 0.01）,and they were decreased significantly after the 36 h in the intervention group,and lower than sepsis group（P〈0.05）.In each time point,TNF-ct and HMGB-1 expression of intervention group and sepsis group were significantly higher than those in the sham operation group（P〈0.05）,and the expression levels were significantly lower in intervention group than in the sepsis group（P〈0.05）,After 36 h,the expression level of TNF-a and HMGB-l in the intervention group and sepsis group were decreased（P〈0.05）.Conclusion Atorvastatin can improve the pathological inflammatory lesion,reduce the serum TNF-α and HMGB-1 expression of lung tissue in rats with sepsis.

关 键 词：阿托伐他汀 脓毒症 肺组织 肿瘤坏死因子-α 高迁移率族蛋白-1 大鼠 

分 类 号：R459.7[医药卫生—急诊医学]