蝙蝠葛诺林碱对家兔体外颈内动脉舒张作用  

Vascular Smooth Muscle Relaxation Effect of Daurinoline on Isolated Rabbit Internal Carotid Artery

作　　者：陈密 卢云[2] 周梅[2] 罗攀[2] 崔海东[2] 郭莲军[2] 

机构地区：[1]湖北省英山县人民医院药剂科,英山438700 [2]华中科技大学同济医学院药理系,武汉430030

出　　处：《医药导报》2015年第2期150-153,共4页Herald of Medicine

摘　　要：目的探讨蝙蝠葛诺林碱对家兔体外颈内动脉血管平滑肌的舒张作用。方法清洁级健康成年家兔,采用机械方法损伤体外血管内皮,通过观察标本对1 mol·L-1乙酰胆碱的舒张反应,以确定血管内皮是否损伤。然后观察蝙蝠葛诺林碱对家兔颈内动脉血管平滑肌的舒张作用及与血管内皮的关系。结果蝙蝠葛诺林碱对甲氧胺、氯化钾及组胺所致的家兔颈内动脉收缩均有明显的扩张作用,在内皮完整的血管,最大舒张分别为89%,90%和80%,而在内皮损伤的血管,最大舒张分别为75%,74%和78%,其作用与血管内皮损伤无明显关系(P>0.05),而对5-羟色胺所致的收缩,其舒张作用具有一定的内皮依赖性,在相同剂量,对内皮完整血管的舒张作用比内皮损伤血管要明显,两者间差异有统计学意义(P<0.05)。结论蝙蝠葛诺林碱能有效舒张家兔体外颈内动脉血管平滑肌,其舒张作用无明显的血管内皮依赖性。Objective To examine the effect of daurinoline on endothelial-dependent and-independent vasorelaxation in the rabbit internal carotid arterial （ICA） ring. Methods Internal carotid artery was isolated from specific pathogen-free adult rabbits. The blood vessel endothelium was injured by mechanical method. Diastole reaction of the sample to 1mol· L 1 acetyl choline was observed to determine injury of the blood vessel endothelium. Relaxant effect of daurinoline on internal carotid artery smooth muscle was detected. Results Daurinoline significantly relaxed internal carotid artery constricted by methoxamine, KC1 and 5-histamine. In the aortic rings with intact endothelium, maximal relaxation was 89% , 90% and 80% , respectively, and the maximal relaxation was 75% , 74% and 78% in isolated aortic rings with endothelium injury, respectively. The relaxant effect had no relationship with endothelium injury （P 〉 0. 05 ）. However, in 5-hydroxytryptamine induced constriction, the relaxant effect of daurinoline was associated with integrity of endothelium ; the relaxant eifect of the same dose of daurinoline was significantly greater on artery with intact endothelium than on artery with endothelium injury （P〈0.05）. Conclusion Daurinoline has relaxation effect on rabbits internal carotid artery smooth muscle. The relaxation action is not obviously associated with the endothelium integrity.

关 键 词：蝙蝠葛诺林碱 动脉 颈内 血管内皮 舒张作用 

分 类 号：R965[医药卫生—药理学]