超高效液相色谱法测定半夏不同炮制品中核苷类成分含量  

Determination of the Content of Nucleosides in Differently Processed Pinellia ternata by UPLC

作　　者：何丹[1] 杨林[2] 秦少容[3] 张景勍[1] 

机构地区：[1]重庆医科大学药学院,重庆400016 [2]重庆医药高等专科学校药学系,重庆401331 [3]太极集团重庆涪陵制药厂有限公司,重庆408000

出　　处：《医药导报》2015年第2期231-234,共4页Herald of Medicine

基　　金：国家科技支撑计划(2011BAI13B03)

摘　　要：目的建立以超高效液相色谱（UPLC）法测定不同炮制工艺半夏中尿苷、鸟苷和腺苷含量的方法。方法色谱柱为ACQUITY UPLC BEH C18（2.1 mm×50 mm,1.7μm）,流动相为水-甲醇,梯度洗脱,柱温30℃,流速0.5 m L·min-1,检测波长254 nm。结果尿苷在2.214～22.140μg·m L-1浓度范围内线性关系良好,回归方程为：Y=93 991X-1 653（r=0.999 9）,平均回收率为99.18%,RSD=2.40%;鸟苷在1.212～12.120μg·m L-1浓度范围内线性关系良好,回归方程为：Y=61 542X-806（r=0.999 9）,平均回收率为98.88%,RSD=1.16%;腺苷在0.245 2～2.452 0μg·m L-1浓度范围内线性关系良好,回归方程为：Y=14 994X-145（r=0.999 9）;平均回收率为98.66%,RSD=0.97%。结论该方法准确、快速,能有效地测定不同炮制工艺半夏中尿苷、鸟苷和腺苷的含量。Objective To establish an ultra-peffonance liquid chromatography （UPLC） method for detecting content of uridine, guanosine and adenosine in Pinellia ternata. Methods Acquity UPLC Ben C18（2.1 mm×50 mm, 1.7μm） was used. The mobile phase was methanol and water by gradient elution mode, and the column temperature was 30 ℃ The flow rate was 0. 5 mL·min ] and the detection wavelength was 254 nm. Results The linear range of uridine was 2. 214 - 22.14 μg·mL-1. The regression equation was Y=93 991 X-I 653 （r=0. 999 9）. The linear range of guanosine was 1. 212- 12.12μg·mL-1 , and the regression equation was Y = 61 542 X- 806 （ r = 0. 999 9）. The linear range of adenosine was 0. 245 2-2. 452 0 μg·mL-1 , and the regression equation was Y = 14 994X- 145 （ r = 0. 999 9 ）. The average recovery was 99.18% （ RSD=2.40% ）, 98.88% （ RSD = 1. 16% ） and 98.66% （ RSD = 0.97% ） for uridine, guanosine and adenosine, respectively. Conclusion The method is rapid, reliable and can be used to determine nucleosides in Pinellia ternate.

关 键 词：半夏 炮制工艺 核苷 色谱法 超高效液相 

分 类 号：R282.71[医药卫生—中药学] R927.2[医药卫生—中医学]