新型隐球菌vps41Δ氮源饥饿反应的数字基因表达谱分析  

Digital Gene Expression Profiling of vps41Δ of Cryptococcus neoformans Under Nitrogen Starvation

作　　者：刘晓光[1] 李伟伟 叶狄[1] 

机构地区：[1]工业发酵微生物教育部重点实验室,天津市工业微生物重点实验室,天津科技大学生物工程学院,天津300457

出　　处：《天津科技大学学报》2015年第1期14-18,共5页Journal of Tianjin University of Science & Technology

基　　金：国家自然科学基金资助项目(31070125);天津市自然科学基金资助项目(11JCYBJC26300)

摘　　要：VPS41基因在新型隐球菌的饥饿反应、巨噬细胞中存活以及致病性等过程中起关键作用.为了进一步研究VPS41介导的饥饿反应信号通路相关基因,利用数字基因表达谱技术检测了在氮源饥饿条件下隐球菌野生型菌株KN99α与饥饿反应缺陷菌株vps41Δ呈显著差异表达的基因.结果表明:在氮源饥饿条件下野生型与vps41Δ中呈显著表达差异的基因共有241个,其中vps41Δ中表达上调的基因有176个,表达下调的基因有65个.差异表达的基因主要涉及细胞的形态结构形成、细胞循环、次级代谢产物合成、能量代谢等生物过程.以上结果为迅速克隆鉴定隐球菌VPS41饥饿信号通路的其他关键基因奠定了基础.VPS41 gene plays an essential role in the starvation response,survival inside macrophages and pathogenicity of Cryptococcus neoformans. To identify other genes involved in the VPS41-mediated starvation response,digital gene expres-sion profiling（DGE）technology was used to determine the differentially expressed genes between the wild strain KN99αand the starvation response defective mutant vps41Δunder nitrogen starvation conditions. The results show that 241 genes in total exhibited significant differences in transcript levels,with 176 genes showing increased expression and 65 genes de-creased expression in the vps41Δmutant strain. These differentially expressed genes were mainly involved in cell morphol-ogy formation,cell cycle regulation,secondary metabolite synthesis,energy metabolism and other biological processes. The above results have provided a valuable list of candidate genes for rapid cloning and characterization of genes participating in the VPS41 starvation signaling pathway of C. neoformans.

关 键 词：新型隐球菌 数字基因表达谱 差异表达基因 VPS41信号通路 

分 类 号：Q756[生物学—分子生物学]