费氏丙酸菌的固定化方法及其发酵丙酸的工艺条件研究  

Immobilization of Propionibacterium freudenreichii and the Processing Condition of its Propionic Acid Fermentation

作　　者：马志刚[1] 王亚军[1] 王德培[1] 

机构地区：[1]天津科技大学生物工程学院,天津300457

出　　处：《天津科技大学学报》2015年第1期25-28,共4页Journal of Tianjin University of Science & Technology

摘　　要：为提高丙酸发酵效率,进行了固定化费氏丙酸菌发酵丙酸的研究.采取吸附-包埋结合法,先以麸皮进行吸附,再用海藻酸钠-聚乙烯醇进行包埋制备固定化细胞.通过正交实验确定了固定化细胞的工艺条件:菌液与麸皮比例为5,m L∶0.6,g,吸附时间为15,min,包埋剂选用海藻酸钠和聚乙烯醇混合物,质量比为3∶3,此条件下的包埋效率达到96.86%.初步研究了碳源及其质量浓度对固定化细胞生产丙酸的影响,确定了以乳酸钠为碳源,质量浓度为40,g/L,丙酸产量可达13.75,g/L.In order to improve the efficiency of propionic acid fermentation,propionic acid fermentation with immobilized Propionibacterium freudenreichii were investigated. Adsorption-entrapping method was used to prepare immobilized cells with bran as the adsorbent and CA-PVA as the entrapping material. Through orthogonal experiment,the propionic acid im-mobilized cells’ fermentation process conditions were determined. The proportion of bacterium solution to bran was 5,mL∶0.6 g,the adsorption time was 15,min,the entrapping agent was the mixture of CA and PVA,the mass ratio was 3∶3. Under this immobilization conditions,the encapsulation efficiency reached 96.86%. The effect of the carbon sources and their con-centration on propionic acid production were investigated. The experimental results showed that the sodium lactate was a better carbon source.When the sodium lactate concentration was 40,g/L,the yield of propionic acid reached 13.75,g/L.

关 键 词：费氏丙酸菌 固定化 载体 丙酸 工艺条件 

分 类 号：Q93[生物学—微生物学]