Stat3调控MMP9和TIMP1的转录与表达  

Regulating the Transcription and Expression of Matrix Metalloproteinase-9(MMP9) and the Tissue Inhibitor of Metalloproteinase 1 (TIMP1) with Stat3

作　　者：颜廷宝[1] 廖兴华[1] 周浩[1] 王楠[1] 张同存[1] 

机构地区：[1]天津科技大学生物工程学院,天津300457

出　　处：《天津科技大学学报》2015年第1期29-33,40,共6页Journal of Tianjin University of Science & Technology

基　　金：国家自然科学基金资助项目(31270837);教育部"长江学者和创新团队发展计划"资助项目(IRT1166)

摘　　要：通过脂质体瞬时转染Stat3重组质粒的方法在NIH3T3细胞中过表达Stat3;采用RT-PCR,Western blot以及荧光素酶活性分析的方法评估Stat3对MMP9及对其内源性抑制因子TIMP1的影响.结果表明:Stat3可增强MMP9及TIMP1基因启动子的活性,促进两者的转录与表达;并且Stat3对TIMP1的促进作用明显强于对MMP9的促进作用,从而使ECM的降解能力减弱,细胞外基质成分积累,导致纤维化的发生.Gene Stat3 was overexpressed in NIH3T3 cells by lipofection transfection,and the effect of Stat3 on the expres-sion of MMP9 and its inhibitor TIMP1 was estimated by RT-PCR,Western Blot and Luciferase Assay. The results show that Stat3 is able to upregulate the transcription and expression of MMP9 and TIMP1 by enhancing their promoter activities and the enhancement of TIMP1 expression induced by Stat3 is stronger than MMP9. It suggests that overexpression of Stat3 may inhibit the degradation of ECM in NIH3T3 cells and promote the development of fibrosis.

关 键 词：信号转导与转录激活因子3 金属蛋白酶9 组织金属蛋白酶抑制因子1 细胞外基质 转录调节 

分 类 号：Q78[生物学—分子生物学]