抑制茶饼病菌放线菌及细菌的筛选与鉴定  

Screening and Identification of Actinomycete and Bacteria Strains with Inhibition Function Against Exobasidium vexans

作　　者：韦思梅 彭丽娟[2,3] 

机构地区：[1]贵州省安龙县农业局,贵州安龙552499 [2]贵州大学贵州山地农业病虫害重点实验室,贵州贵阳550025 [3]贵州大学农学院,贵州贵阳550025

出　　处：《贵州农业科学》2015年第1期73-76,80,共5页Guizhou Agricultural Sciences

基　　金：贵州省农业科技攻关计划项目"茶树重要病虫害防除技术研究与推广"[黔科合NY字(2010)3026];贵州大学大学生创新性实验计划项目"抑制茶饼病菌放线菌;细菌的筛选及鉴定"[贵大创字(2011)043]

摘　　要：为筛选出用于生产上防治茶饼病的生防菌,克服化学农药防治造成的农药残留问题,采用平板稀释和喷雾法,从茶树根际土壤中筛选抑制茶饼病菌的拮抗放线菌和细菌,结合形态学特征与ITS序列测定的方法对拮抗菌进行鉴定。结果表明:从采集土样中共分离到59株放线菌和19株细菌,从中各筛选出1株对茶饼病菌具有拮抗作用的放线菌(C6)和细菌(B-9),平均抑菌圈直径分别为27.00mm和32.00mm;经鉴定,C6为酸疮痂链霉菌(Streptomyces acidiscabies),B-9为枯草芽孢杆菌(Bacillus subtilis)。The antagonism actinomycete and bacteria strains against E.vexans were screened from tea rhizoaphere soil by plate dilution and spraying methods and then identified by ITS sequence analysis based on the morphological characteristics to solve pesticides residues in control of tea blister blight.The results showed that antagonism actinomycete C6 strain with 27.00 mm of average inhibition zone and bacterium B-9strain with 32.00 mm of average inhibition zone is screened from 59 actinomycete strains and 19 bacterium strains isolated from tea rhizoaphere soil respectively.The antagonism actinomycete C6 strain and bacterium B-9strain is identified as Streptomyces acidiscabies and Bacillus subtilis respectively.

关 键 词：茶饼病 生物防治 ITS 形态学特征 

分 类 号：S476.19[农业科学—农业昆虫与害虫防治]