星形胶质细胞在降钙素中枢镇痛机制中的作用  

ROLE OF ASTROCYTE IN CALCITONIN SUPRASPINAL MECHANISM OF ANALGESIC ACTION

作　　者：李海英[1] 肖智[2] 李树法[3] 向菲 王青[1] 

机构地区：[1]贵阳市第一人民医院内分泌科,贵阳550002 [2]遵义医学院医学与生物学研究中心,遵义563003 [3]临沂市人民医院内分泌科,临沂276000 [4]贵阳市第二人民医院内分泌科,贵阳550081

出　　处：《中国疼痛医学杂志》2015年第1期33-37,共5页Chinese Journal of Pain Medicine

基　　金：贵州省贵阳市科技计划项目(2011103)

摘　　要：目的:观察降钙素(s CT)对神经病理性疼痛大鼠中脑导水管周围灰质(PAG)星形胶质细胞D-丝氨酸(D-Ser)释放的影响,明确星形胶质细胞在s CT镇痛中作用机制。方法:72只雄性SD大鼠建立坐骨神经结扎神经病理性疼痛大鼠模型后,随机分为6组(n=12):正常组、CCI组、s CT组、射氟代柠檬酸(FCA)组、苯甲酸钠(SB)组和7-氯犬尿酸(CK)组。除正常组及CCI组外大鼠均腹腔内s CT。von Frey纤维检测其机械痛阈值(MWT),ELISA法检测其PAG中D-Ser水平。结果:建模后大鼠MWT下降,给予腹腔注射s CT后,D-Ser水平和MWT显著上升(P均<0.001);PAG注射FCA或SB,分别引起s CT治疗大鼠D-Ser水平和MWT下降或上升(P均<0.001);PAG注射CK引起s CT治疗大鼠MWT下降(P<0.001),D-Ser水平无变化。结论:s CT引起神经病理性疼痛大鼠PAG星形胶质细胞活化,D-Ser释放增加,增强NMDA受体功能产生镇痛作用。Objective：To observe the astrocyte activation,D-serine release in midbrain periaqueductal gray （PAG） of neuropathic pain rats after intraperitoneal injection of salmon calcitonin （sCT） and elucidate the role of astrocytes activation in the analgesic mechanism of sCT.Methods：Male Sprague-Dawley （SD） rats neuropathic pain model were established on the right sciatic nerve chronic constriction injury （CCI） and randomly divided into 6 groups （n =12）：normal,CCI,sCT,fluorocitrate （FCA）,sodium benzoate （SB） and 7-chlorokynurenate （CK）.Rats of all groups had a intraperitoneal injection of sCT except normal and CCI groups.Mechanical withdrawal thresholds （MWT） were tested by electronic von Frey anesthesiometer and levels of D-serine were measured by ELISA assay.Results：In CCI group,the hind-paw MWT was decreased gradually compared with normal group （P ＜ 0.001）.SB （150 μg/0.3 μl） could significantly elevated MWT （P ＜ 0.001） and D-serine level in sCT-treated rats （P ＜ 0.001）.But the antinociceptive effect of sCT was attenuated significantly by FCA （1.2 μg/0.3 μl）and CK （3.6μg/0.3 μL） pretreatment （P ＜ 0.001）.The D-serine level in sCT-treated rats decreased or unchanged by FCA or CK,respectively.Conclusions：sCT produces a long lasting analgesic effect on neuropathic pain via potentiating the function of astrocyte and increases D-serine level in the PAG of neuropathic pain rats.

关 键 词：降钙素 星形胶质细胞 D-丝氨酸 中脑导水管周围灰质 神经病理性疼痛 

分 类 号：R402[医药卫生—临床医学]