犬细小病毒感染对MDCK细胞增殖活性及ATP酶的影响  

The Infection of the Canine Parvovirus(CPV)on Activities of MDCK Cell Proliferation Activity and ATPase

作　　者：任常宝 许冬蕾 张晓战[3] 黄红亮 唐兆新[1,2,3] 陈瑞爱[1,2,3] 

机构地区：[1]肇庆大华农生物药品有限公司,广东肇庆526238 [2]农业部动物疫病防控生物技术与制品创新重点实验室,广东肇庆526238 [3]华南农业大学兽医学院,广东广州510642

出　　处：《中国兽医杂志》2015年第1期34-37,共4页Chinese Journal of Veterinary Medicine

摘　　要：为了研究犬细小病毒感染对MDCK细胞增殖活性及能量代谢的影响,建立了犬细小病毒感染模型,并于病毒感染后6、12、18、24、30 h和36 h,观察细胞的生长状态,同时检测细胞增殖能力和ATP酶活性。与对照细胞相比,病毒感染细胞在24、30、36 h时增殖活性明显下降(P<0.05)。The purpose is to study the affect of canine parvovirus infection on MDCK cell proliferation and energy metabolism. A model of canine parvovirus infection was established, simultaneously the cell growth status was observed, proliferative ability and the ATP enzymes activity were measured at 6 h, 12 h, 18 h, 24 h, 30 h and 36 h after infection. Compared to control cells, the proliferative activity of virus-infected cells was significantly decreased（P〈0.05 ）at 24 h, 30 h, 36 h after infection.

关 键 词：犬细小病毒 MDCK细胞 潜伏感染 细胞增殖活性 ATP酶 

分 类 号：S852.655[农业科学—基础兽医学]