CODEHOP法设计简并引物克隆鲫鱼肌钙蛋白C(TnC)基因及序列分析  

CODEHOP Method Design Degenerate Primers Cloning and Characterization of Troponin C gene from Carp

作　　者：卢玉婷[1] 张雅斌[2] 王建超[1] 吉尚雷[1] 杜晓燕[2] 张培军[3] 李月红[1] 

机构地区：[1]吉林农业大学动物科学学院,吉林长春130118 [2]吉林省水产科学研究院,吉林长春130000 [3]吉林省卫生监测检验中心,吉林长春130000

出　　处：《中国兽医杂志》2015年第1期91-93,96,共4页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金(30972191);农业部948项目(20-14Z43);吉林省教育厅"十二五"科学技术研究重点项目

摘　　要：本文结合生物信息学资源和生物软件的使用,针对特定的氨基酸序列设计简并引物,利用CODEHOP法设计简并引物克隆鲫鱼肌钙蛋白C(Tn C)基因,并构建系统进化树。证明该基因片段与其他鱼类Tn C基因具有较高的同源性,其中与斑马鱼同源性最高达到96%,与大西洋鲑、白斑狗鱼、虹鳟、斜带石斑鱼、斑点叉尾鮰的同源性分别为92%、92%、91%、91%、85%。研究表明,应用此种方法设计出的引物简并度相对较低,Tm值的修改较为灵活,产物特异性强。We designed degenerate primers for a specific amino acid sequence, combining bioinformatics resources with the biology software. CODEHOP method was used to design degenerate primers to clone carp troponin C （TnC）gene and to construct phylogenefic trees. This paper proved that the gene fragments showed high homology to other fish TnC genes and revealed the highest homology of 96% with Danio rerio , and 92%, 92%, 91%, 91%, 85% compared with Salmo salar , Esox lucius, Oncorhynchus mykiss , Epinephelus coioides , Ictalurus punctatus homologous respectively.The results indicated that degeneracy rate of these degenerate primers was relatively low, Tm value modification was more flexible, and could be used to obtain specific RT-PCR products.

关 键 词：鲫鱼 简并引物 肌钙蛋白C(TnC) 

分 类 号：Q789[生物学—分子生物学] S917[农业科学—水产科学]