RP-HPLC法测定术苦芩颗粒中黄芩苷和苦参碱含量  

Determination of Content of Baicalin and Matrine in Zhukuqin Granules by RP-HPLC

作　　者：邱洪[1] 朱兆荣[1] 刘娟[1] 凌榕镔[1] 罗艺晨[1] 

机构地区：[1]西南大学荣昌校区动物医学系,重庆荣昌402460

出　　处：《中国兽医杂志》2015年第1期97-100,共4页Chinese Journal of Veterinary Medicine

基　　金：国家公益性行业(农业)科研专项(201303040-05);国家自然科学基金资助项目(31172357)

摘　　要：为了建立测定术苦芩颗粒中黄芩苷、苦参碱含量的反相高效液相色谱法,采用Wonda Sil?C18柱(4.6 mm×250 mm,5μm)色谱柱,黄芩苷以甲醇-0.4%磷酸溶液(用三乙胺调节p H值至3.0)(52∶48,V/V)为流动相,检测波长为274 nm。苦参碱以乙腈∶甲醇∶0.2%磷酸溶液(用三乙胺调节p H值至7.0)(22∶13∶65,V/V)为流动相,流速为1.0 m L/min,检测波长220 nm;柱温30℃。术苦芩颗粒中黄芩苷和苦参碱分别在浓度为10μg/m L^150μg/m L和10μg/m L^400μg/m L范围内与峰面积均呈良好线性关系,r=0.99991和r=0.99994,平均加样回收率分别为99.32%和98.37%,其相对标准偏差(RSD)分别为0.625%和1.206%。用上述方法分别测定黄芩苷和苦参碱含量条件简单、稳定、快速,可用于检测术苦芩颗粒的质量控制标准。In order to establish a method for the determination of baicalin and matrine content in Zhukuqin granules. Chromatography was performed on a Wonda Sil C 18 column（ 4.6 mm×250 mm05 μm）. and acetonitrile-methanol-0.2% phosphoric acid solution （V： V = 52：48） as the mobile phase of baicalin was used at the wavelength of 274 nm and acetonitrile-methanol-0.2% phosphoric acid solution （V：V：V=22：13 ： 65） as the mobile phase of matrine was used at the wavelength of 220 nm with 10 IzL of the injection volume, at the flow rate of 1.0 mL/mL and column temperature of 30 ~C.The results showed that the calibration curve of baicalin was linear i.n the rang of 10 trg/mL ～ 150 μg/mL（r=0.99991 ）. and the average recovery rate was 99.32%（RSD=0.625%）. And the calibration curve of matrine was linear in the rang of 10 μg/mL ～400 μg/mL（r=0.99994）. and the average recovery rate was 98.37%（RSD=1.206%）. The method was feasible and stable for the determination of baicalin and matrine content in ZhuKuqin granules as the quality control standard.

关 键 词：反相高效液相色谱法 术苦芩颗粒 黄芩苷 苦参碱 

分 类 号：R286[医药卫生—中药学]