52株侵袭性肺炎链球菌血清型分布及耐药性分析  

Study of serotype distribution and antimicrobial resistance in 52 isolates of invasive Streptococcus pneumoniae

作　　者：林祥芳[1] 刘彩霞[1] 杨海蔚[1] 李方去[1] 李向阳[1] 杨锦红[1] 

机构地区：[1]温州医科大学附属第二医院检验科,浙江温州325000

出　　处：《中国卫生检验杂志》2015年第2期281-283,共3页Chinese Journal of Health Laboratory Technology

基　　金：卫生部国家临床重点专科开放课题(20130101)

摘　　要：目的了解本院临床分离的侵袭性肺炎链球菌的血清型分布及耐药情况,为临床用药提供参考。方法采用荚膜肿胀试验检测2009年1月-2013年5月临床分离的52株侵袭性肺炎链球菌的血清型,采用最小抑菌浓度(MIC)稀释法检测药物敏感性。结果 52株侵袭性肺炎链球菌共鉴定出15个血清型/群,常见的血清型有14(21.2%)、19A(15.4%)、6B(11.5%)、19F(9.6%)、23F(9.6%)、3(9.6%)、6A(5.8%),其中有1株用此方法无法确定血清型;所有的侵袭性肺炎链球菌对万古霉素和左氧氟沙星均敏感,红霉素、四环素、氯林可霉素的耐药率分别为96.2%、64.7%、89.5%。结论本院临床分离的侵袭性肺炎链球菌的血清型主要集中在14、19A、6B、19F、23F、3、6A,对红霉素、四环素、氯林可霉素等耐药情况较严重。Objective To investigate the serotype distribution and antibiotic resistance of clinical isolates of invasive Streptococcus pneumoniae in our hospital,and to provide reference for clinical medication and vaccine research. Methods A total of 52 strains invasive Streptococcus pneumoniae were collected from January,2009 to May,2013,serotypes of Streptococcus pneumoniae were performed by using quelling reaction. MIC dilution method was used to detect drug sensitivity. Results 15 serum type / group were identified in 52 strains of invasive pneumoniae, the most common serotypes were 14（ 21. 2%）,19A（ 15. 4%）,6B（ 11. 5%）,19F（ 9. 6%）,23F（ 9. 6%）,3（ 9. 6%）,6A（ 5. 8%）,1 strain could not be determined for serotype. All invasive Streptococcus pneumoniae were sensitive to vancomycin and levofloxacin,erythromycin,tetracycline,clindamycin,and the resistant rates were 96. 2%,64. 7% and 89. 5% respectively. Conclusion The major serotypes of invasive Streptococcus pneumoniae were 14,19 A,6B,19 F,23F,3,6A,which were seriously resistant to erythromycin,tetracycline and clindamycin.

关 键 词：侵袭性肺炎链球菌 血清型 耐药性 分布 

分 类 号：R446.5[医药卫生—诊断学]