医用细菌纤维素的制备及其细胞相容性  

Production and Cell Compatibility of Medical Bacterial Cellulose

作　　者：李立风[1,2] 任力[1,2] 刘卅[1,2] 王迎军[1,2] 

机构地区：[1]华南理工大学材料科学与工程学院,广东广州510640 [2]华南理工大学国家人体组织功能重建工程技术研究中心,广东广州510006

出　　处：《华南理工大学学报（自然科学版）》2014年第10期44-49,56,共7页Journal of South China University of Technology(Natural Science Edition)

基　　金：国家自然科学基金资助项目(50803018;51273072);广东省科技计划项目(2012A080203010;2012A080800015)

摘　　要：以椰汁作为基本的碳源和生长因子来源,通过添加成分明确的乙醇及酵母膏作为补充碳源和氮源,并补加相应的微量元素,获得了成分简单、明确的医用细菌纤维素培养基;该培养基各成分含量相对稳定,提高了细菌纤维素的发酵速率和产量,所获得的产物厚度可满足不同的医用需求.将碱液分别与十二烷基硫酸钠(SDS)、超声波及乙醇联用对发酵产物进行纯化处理.结果表明:碱液与SDS联用时纯化效果最好;纯化所得样品的杂质N含量低于检测限,透光率高,在可见光范围内与活体角膜透光率相近,L929细胞相容性良好.A medical bacterial cellulose medium with simple and definite components was obtalneu t^y aamng trace elements with coconut juice as the basic carbon source and the growth factor source, yeast extract as the nitrogen source and ethanol as the addition carbon source. The medium contains components with relatively stable content and can improve the fermentation rate and the yield of bacterial cellulose, and the product thickness can meet vari- ous medical requirements. Then, alkali is used to purify the fermentation product by combining SDS or ultrasound or ethanol. The results show that alkali and SDS treatment procedure has the best purification effect, and that the purified sample possesses a content of impurity nitrogen below the detection limit and a high transmittance which is close to cornea in vivo in the visible region, and it exhibits a good biocompatibility with L929 cells.

关 键 词：细菌纤维素 培养基 纯化 细胞相容性 医用材料 

分 类 号：R318.08[医药卫生—生物医学工程]