帽天山、蛟龙潭土壤微生物多样性的Biolog-ECO分析  

Analysis of soil microbial diversity by Biolog-ECO technology in Maotianshan and Jiaolongtan

作　　者：刘云华[1] 吴毅歆[1,2] 刘春明[1] 何鹏飞[1] 何月秋[1,2] 

机构地区：[1]云南农业大学农业生物多样性与应用技术国家工程中心,昆明650201 [2]微生物菌种筛选与应用国家地方联合工程研究中心,昆明650217

出　　处：《生态科学》2014年第6期1047-1052,共6页Ecological Science

基　　金：国际科技合作项目(2009DFA32360);昆明市科技局项目(09H130301)

摘　　要：利用Biolog-ECO技术,研究了澄江县帽天山和蛟龙潭磷矿区修复后土壤微生物功能多样性。结果表明,帽天山土壤微生物对碳源的利用能力明显高于蛟龙潭,土壤微生物群落种类亦相对较丰富,而蛟龙潭土壤微生物群落功能多样性相对较丰富。两地区对6类碳源的优先利用顺序也不同,帽天山地区依次为:氨基酸类、多聚物类、碳水化合物类、胺类、酚酸类、羧酸类碳源;蛟龙潭地区依次为:氨基酸类、碳水化合物类、多聚物类、酚酸类、羧酸、胺类碳源。两地区土壤主要微生物类群也存在差别:帽天山以利用多聚物类,碳水化合物类,氨基酸类和胺类为主要碳源的土壤微生物群落为其主要类群;蛟龙潭以利用多聚物类,酚酸类,氨基酸类和胺类为主要碳源的土壤微生物群落为其主要类群。By using Biolog-ECO technology, the microbial diversity was studied in the soil from phosphorus-mining regions of Maotianshan （MST） and Jiaolongtan（JLT）, Chengjiang County. The results showed that the ability of the microbe to use carbon sources and microbial community was significantly higher and richer, respectively, in MST than in JLT, but JLT had higher soil microbial community functional diversity. The two types of microbes from MST and JLT were quite different in the priority to use the 6 carbon sources： amino acids, polymers, carbohydrates, amines, phenolic acids, carboxylic acids for MST and amino acids, carbohydrates, polymers, phenolic acids, carboxylic acids, amines for JLT in order. The main microbial types were those to use polymers, carbohydrates, amino acids and amines in MST and polymer type, phenolic acids, amino acids and amines in JLT as major carbon sources.

关 键 词：土壤微生物群落 碳源利用 主成份分析 丰富度指数 代谢能力 

分 类 号：S154.3[农业科学—土壤学]