深圳地区儿童腹泻A组轮状病毒检测和基因型分析  

Detection and Genotyping of Group A Rotavirus from Children with Diarrhea in Shenzhen

作　　者：王琼[1] 张银辉[1] 何涛君[1] 黄烈[1] 杨来智[1] 吴润香[1] 陆学东[1] 

机构地区：[1]广东医学院附属福田医院检验医学部,广东深圳518033

出　　处：《现代检验医学杂志》2014年第6期84-86,90,共4页Journal of Modern Laboratory Medicine

摘　　要：目的：了解深圳地区5岁以下腹泻患儿 A组轮状病毒的感染状况及其基因型特点。方法收集深圳市福田人民医院2007年8月～2009年2月腹泻患儿粪便标本413份，采用反转录-聚合酶链反应（RT-PCR）法检测 A组轮状病毒基因，阳性者再采用巢式 RT-PCR 分别针对病毒 VP7，VP4基因保守区进行 G／P型别检测。结果413份粪便标本 A组轮状病毒阳性率为52.5％（217／413），轮状病毒腹泻在深圳的主要流行季节是10～次年1月份，2岁以内的患儿占全部患儿总数的91.2％（198／217）。对217份轮状病毒阳性样本进行G／P分型，流行的轮状病毒G型分型依次为G3（52.1％），G1（31.3％），混合 G型感染（6.0％），G2（1.8％）及 G9（1.4％）；P型分型依次为 P[8]（85.7％），P[4]（6.0％）及混合 P型感染（1.4％）；G型和P型的组合以 G3P[8]（47.5％）最为常见，其次为 G1P[8]（30.4％）。结论 A组轮状病毒是深圳地区婴幼儿腹泻的主要原因，G3P[8]和 G1P[8]是2007年～2009年深圳地区的主要轮状病毒流行株，此外混合型并不少见。Objective To investigate the prevalence and genotype characteristics of group Arotavirus among children under 5 years of age with diarrhea in Shenzhen.Methods 413 fecal specimens were collected from children with acute diarrhea in Futian hospital from August 2007 to February 2009 in Shenzhen.Reverse transcription PCR（RT-PCR）were used to detect group A rotavirus.G and P genotyping were conducted to the positive specimens by using nested RT-PCR to amplify VP7 and VP4 gene.Results Group A rotavirus was detected in 217 of 413 specimens with the positive rate of 52.5%.The main epidemic season for rotavirus was from October to January in Shenzhen.The majority of children suffered rotavirus were un-der 2 years of age which account for 91.2% （198/217）of all rotavirus infections.The prevalence of G genotype was G3 （52.1%）,G1 （31.3%）,Gmix （6.0%）,G2 （1.8%）and G9（1.4%）respectively.The prevalence of P genotype was P[8] （85.7%）,P[4]（6.0%）and Pmix （1.4%）respectively.G3P[8]and G1P[8]were the most common G and P genotype combinations accounting for 47.5% and 30.4% of rotavirus infections,respectively.Conclusion Group A rotavirus was the major pathogen of diarrhea among children in Shenzhen.G3P[8]and G1P[8]were the predominant genotypes from 2007 to 2009.G mix and P mix genotype were not uncommon in this region.

关 键 词：轮状病毒 腹泻 儿童 基因分型 

分 类 号：R373.25[医药卫生—病原生物学] Q786[医药卫生—基础医学]