实验室纯水系统更换反渗透膜对生化检验的影响  

Effect of Replacement of New Reverse Osmosis Film of Water Purification System to Clinical Biochemistry

作　　者：莫基浩[1] 刘鲜茹[1] 李少侠[1] 

机构地区：[1]河南省洛阳正骨医院检验中心,河南洛阳471000

出　　处：《现代检验医学杂志》2014年第6期124-126,130,共4页Journal of Modern Laboratory Medicine

摘　　要：目的：研究实验室纯水系统更换新反渗透膜后，所产纯水的质量是否符合生化检验的要求并评价其影响。方法通过Beckman Coulter生化分析系统配套纯水机天创TCHS-RO／100A自带电阻表和 HM COM-100电导率测量笔连续监测新产纯水中的实时离子含量。用正常纯水在 AU680上重复20次测量 ALT，AST，TBIL，ALP，GGT，TP，ALB，BUN， CR，UA，GLU，TC，TG，HDL，LDL，CK，LDH，P，Ca（对照组），更换为新产纯水后进行相同试验操作（试验组），以评估新产纯水对生化分析的影响。连续5天，每天3次（间隔2 h）监测新产纯水的 TG试剂空白，监测水中甘油水平。结果开机30 min后，HM COM-100测得电导率降至（0.1±0.0）μS／cm，机载电阻表测得新产纯水在开机50 min后稳定在18.25 MΩcm。在用 AU680进行生化分析时，对照组中TG测量值＝1.04 mmol／L，试验组＝21.39 mmol／L，差异有统计学意义（P＜0.01），而除TG其他生化项目偏倚均在5％以内，CV＜1／4CLIA’88，差异无统计学意义（P＞0.05）。TG试剂空白在第5天稳定在A660nm＝0.0156＆#177;0.0004。结论新纯水系统在生产约2800 L水后，所产纯水中的甘油可以清除干净。实验室纯水系统在更换反渗透膜后，需要对水质进行连续监测，以评价其对常规生化项目检测的影响。Objective To test if the quality of produced water could come up to the standards of clinical biochemistry,and e-valuate the effect of the produced water after replacement of a new reverse osmosis film of the laboratory water purification system.Methods The ion concentration of the produced water was tested by ohmmeter in Beckman Coulter supporting TianChuang water purification system TCHS-RO/100A and HM COM-100 conductivity meter.The biochemical index ALT,AST,TBIL,ALP,GGT,TP,ALB,BUN,CR,UA,GLU,TC,TG,HDL,LDL,CK,LDH,P,Ca of mixed serum were tested 20 times by AU680 under the condition of normal purified water（control group）,and the same operation were repeat-ed after changing to newly produced water（experimental group）to evaluate the effect of newly produced water to biochemi-cal analysis.The triglyceride （TG）reagent blank was tested 3times/d for 5 days to evaluate the glycerin level in the pro-duced water.Results The conductivity of purified water was reduced to （0.1±0.0）μS/cm 30 min after starting up by HM COM-100,and the electric resistance of purified water remained stable at 18.25MΩcm 50min after starting up.During the bi-ochemical analysis by AU680,the value of TG in control group=1.04 mmol/L,in experimental group=21.39 mmol/L,the differences showed statistical significance （P〈0.01）,but the bias of the others were limited in 5%,CV〈1/4CLIA’88,and differences showed no statistical significance （P〉0.05）.The reagent blank was stable at A660nm=0.015 6±0.000 4 in the fifth day.Conclusion After produced approximately 2 800 L purified water in the system,glycerine could be cleaned up in produced water.It is needed to continuously test the quality of produced water to evaluate the effect of which to clinical bio-chemistry after replacement of a new reverse osmosis film.

关 键 词：三酰甘油 纯水 反渗透膜 生化 

分 类 号：R446.112[医药卫生—诊断学]