血清TNF-α和HSP70表达水平与回汉族2型糖尿病关系的研究  

Association of TNF-α and HSP70 in serum with type 2 diabetes mellitus among Hui and Han nationalities in Ningxia

作　　者：陶秀娟[1] 宋琳[2,3] 刘贺荣[1] 张伟[1] 强艳[4] 宋辉[1] 

机构地区：[1]宁夏医科大学公共卫生学院,银川750004 [2]宁夏医科大学第二附属医院 [3]银川市第一人民医院,银川750001 [4]宁夏医科大学总医院,银川750004

出　　处：《军事医学》2014年第12期940-944,共5页Military Medical Sciences

基　　金：国家自然科学基金资助项目(81160110);宁夏自然科学基金资助项目(NZ11109)

摘　　要：目的探讨血清肿瘤坏死因子α（tumor necrosis factor-α,TNF-α）、热休克蛋白70（heat shock protein 70,HSP70）表达水平对宁夏回、汉民族2型糖尿病（type 2 diabetes mellitus,T2DM）发病的影响。方法采用病例对照研究设计,按照纳入与排除标准,于2011年10月至2012年10月在宁夏医科大学第二附属医院与教学医院（吴忠市人民医院）内分泌科确诊的225例T2DM患者及同期健康检查人群中确定的600例健康人作为研究对象。采用双抗体夹心酶联免疫吸附法（enzyme linked immunosorbent assay,ELISA）测定血清中TNF-α与HSP70蛋白表达水平。结果病例组回、汉族合计人群和回族人群血清TNF-α表达水平明显高于对照组（t=-3.512,-3.791,均P〈0.01）;病例组汉族人群TNF-α表达水平及回、汉民族中不同组间HSP70表达水平的差异均无统计学意义（t=-0.565,-0.020,-1.251,-0.809,均P〉0.05）;TNF-α水平高表达人群的发病风险明显高于低表达人群（OR=1.500,95%CI：1.075~2.093,P=0.017）。回族合计人群、回族病例及回族对照人群血清TNF-α表达水平均显著低于汉族合计人群、汉族病例及汉族对照人群,差异均有统计学意义（t=9.373,2.223,9.734,均P〈0.01或0.05）。TNF-α表达水平与血糖水平呈正相关（r=0.110,P=0.001）,HSP70表达水平与血糖水平间无明显相关关系（r=0.023,P=0.504）。多因素Logistic回归分析显示,性别和腰臀比（waist-to-hip ratio,WHR）为TNF-α高表达的危险因素（P〈0.01或0.05）,HSP70和民族为保护因素;收缩压（systolic blood pressure,SBP）和WHR为HSP70高表达的危险因素（均P〈0.01）,TNF-α为保护因素（P〈0.05）;HSP70、TNF-α、性别、家族史、BMI、SBP、甘油三酯（triglycerides,TG）、低密度脂蛋白胆固醇（low-density lipoprotein cholesterol,LDL-C）、丙氨酸氨基转移酶（alanine trans-aminase,ALT）、心理应激为T2DM发病的危险因素（P〈0.01或0.05）,高密度脂蛋白胆固�Objective To investigate the expression level of tumor necrosis factor α （TNF-α）, heat shock protein 70 （HSP70） in serum and to analyze the correlation of TNF-α and HSP70 with the incidence of type 2 diabetes mellitus （T＇2DM） among Hui and Han nationalities in Ningxia. Methods Using the method of case-control study and according to the inclusion and exclusion criteria of case and control groups, 225 patients with T2DM who were treated by Department of Endocrinology and 600 healthy people during health checkups were enrolled in the study between October 2011 and October 2012. A double antibody sandwich enzyme-linked immunosorbent assay （ELISA） was used to determine the protein expression levels of TNF-α and HSP70 in serum. Results The expression levels of TNF-α in the total population investigated and Hui population in T2DM group were significantly higher than in the control （ t = - 3. 512, - 3. 791, all P 〈 0.01 ）. The difference in expression levels of TNF-α between the Han people of T2DM group and control group, and that in the expression levels of HSP70 between the case group and control group were not statistically significant （ t = - 0. 565, - 0. 020, - 1.251 ,- 0.809, all P 〉 0.05 ）. The elevated serum levels of TNF-ot increased the risk for T2DM （ OR = 1. 500, 95% CI：I. 075 -2. 093 ,P = 0. 017 ）. The expression levels of TNF-ot in the total people, case and control of Hui people were significant lower than the homologous Han people（ t = 9. 373,2. 223,9. 734, all P 〈 0.01 or 0.05 ）. Pearson analysis showed that the high expression of TNF-α was positively correlated with blood glucose level （r = 0. 110 ,P = 0.001 ）, but there was no correlation between the high expression of HSP70 and blood glucose level （ r = 0. 023, P = 0.504）. The result of multiple logistic regression analysis indicated that gender and waist-to-hip ratio（WHR） were risk factors for high levels of TNF-ot （P 〈 0.01 or 0.05 ）while HSP70 and nationalities were protective facto

关 键 词：糖尿病 2型 肿瘤坏死因子Α HSP70热休克蛋白质类 回族 汉族 

分 类 号：R587.1[医药卫生—内分泌]