温肾生精饮对环磷酰胺致小鼠睾丸氧化损伤的保护作用  

Protective effect of Wenshen Shengjing Decoction on cyclophosphamide induced testicular oxidative damage in mice

作　　者：潘晓燕[1] 李康[1] 王喜艳[2] 王雪楠[3] 孙占轩 严之贞 林寿想 范佳佳[1] 李质馨[1] 

机构地区：[1]吉林医药学院组织学与胚胎学教研室,吉林吉林132013 [2]吉林医药学院医学实验中心,吉林吉林132013 [3]济宁医学院附属医院生殖医学中心,山东济宁272029

出　　处：《军事医学》2014年第12期957-961,共5页Military Medical Sciences

基　　金：吉林省科技发展计划重点攻关项目(20140204033YY);吉林省中医药管理局中医药科技项目(2012-165);吉林市科技发展计划资助项目(2013625023);山东省高等学校科技计划项目(J13LL04);吉林医药学院大学生科研基金[吉医学科字(2012)第03号];2014年吉林省大学生创新创业训练计划项目

摘　　要：目的研究温肾生精饮(主要由鹿茸、人参、锁阳、大云、黄芪、淫羊藿和当归等15味药材组成)对环磷酰胺致小鼠睾丸氧化损伤的保护作用及其机制。方法将8周龄的昆明种雄性小鼠随机分组,正常对照组腹腔注射生理盐水,模型组、西药组和中药组连续5 d腹腔注射80 mg/(kg·d)的环磷酰胺,然后将正常对照组和模型组小鼠灌胃生理盐水,西药组和中药组小鼠分别灌胃克罗米芬或温肾生精饮30 d。检测各组小鼠附睾精子密度和精子活率;观察睾丸生精上皮的发育;检测睾丸组织中丙二醛(MDA)、谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GPH-Px)和过氧化氢酶(CAT)的水平;免疫组织化学法检测睾丸生精小管中生存素(survivin)的表达;TUNEL法检测生精细胞的凋亡。结果与模型组和西药组比较,温肾生精饮显著提高了生精障碍小鼠的精子密度和精子活率,促进了生精小管上皮的发育,显著提高了生精障碍小鼠睾丸内GSH-Px和CAT的活性,降低了睾丸组织中MDA水平,且上调了生精细胞中生存素的表达水平,降低了生精小管和生精细胞的凋亡指数。结论温肾生精饮能显著修复环磷酰胺致小鼠睾丸生精功能损伤,其修复机制可能与提高睾丸组织中的抗氧化水平、减少生精细胞凋亡有关。Objective To investigate the protective effect and the possible mechanism of Wenshen Shengjing Decoction（WSSJD）（including Cornu Cervi Nippon Parvum,Panax ginseng,Cynomorium songaricum,Cistanche deserticola,Radix Astragali,Epimedium brevicornum,Angelica sinensis.） on cyclophosphamide induced testicular oxidative damage in mice.Methods Eight-week-old male Kunming mice were assigned.Mice in normal control group received intraperitoneally normal saline,and mice in Western medicine group,WSSJD group and model group were injected intraperitoneally with cyclophosphamide [80 mg /（kg·d） ]for five days.Then mice in Western medicine group and WSSJD group were gavaged with clomifene citrate or WSSJD for 30 days consecutively.The epididymal sperm count and sperm viability were recorded,and the structure of seminiferous epithelium was observed.The content of malondialdehyde（MDA） and glutathione（GSH）,and activities of glutathione peroxidase（GSH-Px） and catalase（CAT） in testicular tissue were detected.Immunohistochemical technique was used to detect the expression of survivin in spermatogenic cells.The apoptosis of spermatogenic cells in the testes was detected by TUNEL assay.Results Compared with model and Western medicine groups,the epididymal sperm count and sperm viability rate,the development of seminiferous epithelium,and the activities of GSH-Px and CAT significantly increased,while the MDA content significantly decreased in WSSJD group.The expression of survivin in spermatogenic cells was significantly improved.The apoptotic indexes of seminiferous tubules and spermatogenic cells significantly decreased in WSSJD group.Conclusion These results suggest that WSSJD can significantly improve the impaired reproductive function induced by cyclophospamide in mice by enhancing the antioxidative capability in testes and reducing the apoptosis of spermatogenic cells.

关 键 词：温肾生精饮 环磷酰胺 睾丸 氧化损伤 生存素 凋亡 

分 类 号：R285.5[医药卫生—中药学]