河北省藁城花生病毒病的RT-PCR检测及外壳蛋白基因序列分析  

Detection of peanut viruses in Gaocheng of Hebei Province by RT-PCR and sequence analysis of its coat protein gene

作　　者：康彦平[1] 晏立英[1] 雷永[1] 廖伯寿[1] 

机构地区：[1]中国农业科学院油料作物研究所/油料作物生物学与遗传改良重点实验室,湖北武汉430062

出　　处：《中国油料作物学报》2015年第1期102-105,共4页Chinese Journal of Oil Crop Sciences

基　　金：基金项目: 国家花生产业技术体系(CARS-14)

摘　　要：采用RT-PCR技术对采自河北省农林科学研究院粮油作物研究所藁城堤上试验基地的12份花生叶片样品进行花生病毒病的检测,结果表明：从表现花叶和矮化症状的10份样品中检测到花生条纹病毒（peanutstripevirus,PStV）,检出率高达90%,未检测到花生矮化病毒和黄瓜花叶病毒;对其中2个PStV分离物cp基因序列分析的结果表明,该cp全长864bp.这两个分离物与已报道的PStV其他株系的cp核苷酸序列相似性分别为94.4% ～99.2%和94.7% ～99.7%,氨基酸相似性分别为95.5% ～99.7%和95.8% ～100.0%.PStVcp核苷酸序列构建的系统进化树中,这两个分离物与中国大陆其它分离物及美国分离物亲缘关系较近,而与中国台湾、泰国和越南分离物亲缘关系较远.Peanut virus diseases can cause mosaic and dwarf symptoms on peanut plants. and influence peanut's production. Reverse transcription PCR( RT- PCR) was used to detect peanut viruses in 12 peanut samples collected from Gaocheng,Hebei province. Results revealed that only Peanut stripe virus( PSt V) was detected in the 90% of the collected samples with mosaic and dwarf symptom. The coat protein gene( CP) of PSt V of two isolates were sequenced,and their sizes were 864 base pairs( bp). Sequence analysis showed that the sequences of Gaocheng isolates shared 94. 4%- 99. 2% and 94. 7%- 99. 7% similarities at nucleotide( nt) level and 95. 5%- 99. 7% and 95. 8%- 100. 0% at amino acid( aa) level with those of other PSt V strains. These isolates shared closer relationship with Chinese and USA isolates compared with Chinese Taiwan,Thailand and Vietnam isolates by phylogenetic analysis of CP nucleotide sequence.

关 键 词：花生病毒病 RT-PCR CP基因 序列分析 

分 类 号：S435.652[农业科学—农业昆虫与害虫防治]