人G6PD基因启动子区结合蛋白的生物信息学分析  

Bioinformatics Analysis of Transcription Factor Binding Site in the Promoter of Human G6PD Gene

作　　者：卢玲琳 梁海萍[1] 颜冬菁[2] 

机构地区：[1]海南医学院理学院,海南海口571199 [2]海南医学院理学院生化与分子生物学教研室,海南海口571199

出　　处：《生物技术世界》2015年第4期13-14,16,共3页Biotech World

基　　金：国家级大学生创新创业训练计划项目(No.201311810051);海南医学院创新实验计划项目(No.HYCX201218);海南医学院人才引进基金项目(No.2013005)

摘　　要：目的:对人G6PD基因启动子区结合蛋白进行生物信息学分析。方法:从Gene Bank数据库中获取人G6PD基因5’调控区,使用在线软件JASPAR对该序列可能存在的转录因子结合位点进行预测。结果:获得人G6PD基因5’调控区序列2200 bp(-2000^+200 bp)。Relative profile score threshold在85%、90%、95%和100%时,该序列存在805、258、66和17个可能的转录因子结合位点,包含Maf G、Pdx1、Prrx2、MZF1、SPIB、KLF5和SP1等因子。结论:人G6PD基因5’调控区存在多种转录因子的结合位点,这些转录因子大多与胰腺发育、肿瘤发生和氧化应激有关。Objective: To analysis of transcription factor binding site in the promoter of human G6 PD gene. Methods: The 5' regulatory region of G6 PD gene was obtained from Gene Bank database. The transcriptional binding sites of G6 PD gene were predicted by online software JASPAR. Results: The 5'regulatory region of G6 PD gene was 2200 bp(-2000 ~ +200 bp). 805, 258, 66 and 17 potential transcription factors binding sites were predicted in the 5'regulatory region of G6 PD gene when the relative profile score higher than 85%, 90%, 95% and 100%, including Maf G, Pdx1, Prrx2, MZF1, SPIB, KLF5, SP1,et al. Conclusion: There were?many transcription factor?binding sites of?human G6 PD gene 5' regulatory region, which were associated with pancreas development, tumor and oxidative stress.

关 键 词：人G6PD基因 5’调控区 启动子 转录因子结合位点 

分 类 号：Q756[生物学—分子生物学]