Apc^(Min/+)小鼠红细胞形态及参数分析  

An Analysis of Morphology and Parameter of Red Blood Cell in Apc^(Min/+) Mice

作　　者：车轶群[1] 张思维[2] 沈迪[1] 马文韬[1] 曹迎亚[1] 刘婷婷[1] 郝佳洁[1] 蔡岩[1] 王明荣[1] 张钰[1] 

机构地区：[1]中国医学科学院肿瘤医院分子肿瘤学国家重点实验室,北京100021 [2]北京中医药大学东直门医院东区,北京100021

出　　处：《中国肿瘤》2015年第2期160-162,共3页China Cancer

基　　金：国家高技术研究发展规划(863计划项目)(2012AA022403);国家自然科学基金面上项目(81472562)

摘　　要：[目的]探讨结直肠癌小鼠模型Apc Min/+贫血的类型及病因。[方法]对33只Apc Min/+小鼠和23只同窝对照Apc+/+小鼠进行外周血血细胞计数,比较两组之间红细胞计数(RBC)、血红蛋白含量(HGB)、红细胞压积(HCT)、平均红细胞体积(MCV)、平均红细胞血红蛋白量(MCH)和平均红细胞血红蛋白浓度(MCHC)间的差异,以及ApcMin/+小鼠上述各项参数与健康小鼠正常值之间的差异。同时,制备外周血血涂片,观察ApcMin/+小鼠红细胞形态的变化。[结果]ApcMin/+小鼠与对照组Apc+/+小鼠的红细胞参数RBC、HGB、HCT、MCV、MCH和MCHC之间均有显著性差异(P均<0.05)。其中,ApcMin/+小鼠的RBC、HGB、HCT、MCH和MCHC各项均值都显著低于对照组,而其MCV的均值显著高于对照组。与之相似,ApcMin/+小鼠的RBC、HGB、HCT、MCV、MCH和MCHC与健康小鼠相比也存在显著性差异(P均<0.05)。Apc Min/+小鼠的血涂片显示易出现大红细胞、嗜多色红细胞、嗜碱性点彩红细胞及网织红细胞。[结论]与对照组Apc+/+小鼠相比,ApcMin/+小鼠的RBC、HGB、HCT、MCH和MCHC的均值降低,而MCV的均值升高,同时红细胞数目减少,提示其为大细胞低色素性贫血。[Purpose] To investigate the type and pathogenesis of anemia in a colorectal cancer mouse model Apc^Min/＋（multiple intestinal neoplasia）. [Methods] The number of peripheral blood cells,red blood cell count（RBC）,hemoglobin（HGB）,hematocrit（HCT）,mean corpuscular volume（MCV）,mean corpuscular hemoglobin（MCH） and mean corpuscular hemoglobin concentration（MCHC） between Apc^Min/＋mice（n=33） with control mice Apc^＋/＋（n=23） and health mice were analyzed and compared. The morphological changes of blood cells were observed on the blood smear. [Results] There were significant differences between Apc^Min/＋ and Apc^＋/＋mice on red blood cell parameters RBC,HGB,HCT,MCV,MCH and MCHC respectively（all P 0.05）. Among them,RBC,HGB,HCT,MCH and MCHC of Apc^＋/＋mice were significantly lower than those of Apc^＋/＋ mice;however,the mean value of MCV of Apc^Min/＋ mice was higher than that of the Apc^＋/＋ mice. Likewise,there were significantly differences between Apc^Min/＋ mice and healthy mice with regard to RBC,HGB,HCT,MCV,MCH and MCHC（all P0.05）. In addition,there were large erythrocytes,polychromatic erythrocytes,basophilic stippling erythrocytes and reticulocytes on the blood smears of Apc^Min/＋ mice. [Conclusion] Apc^Min/＋ mice exhibited decreases values of RBC,HGB,HCT,MCH,MCHC and increases the number of MCV,indicating Apc^Min/＋ mice have macrocytic hypochromic anemia.

关 键 词：Apc^Min 小鼠模型 贫血 红细胞 

分 类 号：F735.34[经济管理—产业经济]