HPLC-ESI-MS对石榴皮中鞣质类化学成分的初步分析  

Preliminary Analysis of Tannin-related Constituents in Pomegranate Peel by HPLC-ESI-MS

作　　者：周本宏[1,2] 易慧兰 郭咸希[1] 冯琪[2] 刘刚[1] 

机构地区：[1]武汉大学人民医院药学部,武汉430060 [2]武汉大学药学院

出　　处：《中国药师》2015年第2期201-204,共4页China Pharmacist

基　　金：国家自然科学基金资助项目(编号:31170330)

摘　　要：目的:用HPLC-ESI-MS初步鉴定石榴皮提取物中鞣质类化学成分。方法:采用Kromasil C18色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈∶水,梯度洗脱;流速:1.0 ml·min-1,检测波长:256 nm;采用负离子检测模式。结果:初步鉴定出石榴皮提取物中5个鞣质类化学成分,分别为没食子酸,安石榴磷,安石榴苷,鞣云实精,鞣花酸。结论:本方法快速、简便,适合石榴皮中鞣质类化学成分的初步鉴别,为中药活性成分研究提供新的思路。Objective： To identify the tannin-related constituents in the extracts of pomegranate peel by HPLC-ESI-MS. Methods：The separation was performed on a Kromasil C18 column（ 250 mm × 4. 6 mm,5 μm） with the mobile phase of water and acetonitrile.The flow rate was maintained at 1. 0 ml·min- 1and the detection wavelength was set at 256 nm. The samples were analyzed in negative modes. Results： Five tannin-related constituents including gallic acid,punicalin,punicalagin,corilagin and ellagic acid were identified from the extracts of pomegranate peel. Conclusion： The new method is accurate and rapid. It can be used to identify the tannin-related constituents in pomegranate peel and provide new ideas for the research of active components in traditional Chinese medicine as well.

关 键 词：HPLC-ESI-MS法 石榴皮提取物 化学成分 

分 类 号：R284.1[医药卫生—中药学]