LC-MS/MS法同时测定中药白及中militarine、原儿茶酸和咖啡酸的含量  

Simultaneous Determination of Militarine,Protocatechuic Acid and Caffeic Acid in Bletilla Striata by LC-MS/MS

作　　者：陈黎[1] 刘春霞[1] 何秀丽[1] 张秀华[1] 

机构地区：[1]湖北医药学院附属太和医院药学部,湖北十堰442000

出　　处：《中国药师》2015年第2期230-232,共3页China Pharmacist

基　　金：湖北省2011协同创新中心基金(编号:4)

摘　　要：目的:采用Q-TRAP LC-MS/MS测定中药白及中活性成分militarine、原儿茶酸和咖啡酸的含量。方法:采用Supelco Discovery C-18(150 mm×2.1 mm,3μm)色谱柱,以乙腈和水(含体积比0.1%甲酸)为流动相,梯度洗脱,流速为0.25 ml·min-1,AB Sciex 4000 Q-TRAP型质谱仪多重反应监测(MRM)扫描模式对白及对照药材和白及样品分别进行检测。结果:白及对照药材中militarine、原儿茶酸和咖啡酸的含量分别为1.167 5,0.062 6,0.001 0 mg·g-1.白及供试品药材中militarine、原儿茶酸和咖啡酸的含量分别为0.708 8,0.001 1,0.000 4 mg·g-1(n=3)。结论:该方法简便、准确、重现性好,可用于对中药白及中militarine、原儿茶酸和咖啡酸这三个成分进行定量分析。Objective： To develop a Q-TRAP LC-MS / MS method for the content determination of militarine,protocatechuic acid and caffeic acid in Bletilla Striata. Methods： A Supelco Discovery C-18（ 150 mm × 2. 1 mm,3 μm） column was used and the mobile phase was acetonitrile- water containing 0. 1% formic acid（ v / v） with gradient elution. The flow rate was 0. 25 ml·min- 1. Bletilla Striata contrast medicinal materials and Bletilla Striata samples were detected by AB Sciex 4000 Q-MRM scan mode. Results： The content of militarine,protocatechuic acid and caffeic acid in the control herb of Bletilla Striata was 1. 167 5,0. 062 6 mg·g- 1 and 0. 001 0 mg·g- 1,respectively,and that in Bletilla Striata samples was 0. 708 8,0. 001 1 mg·g- 1 and 0. 000 4 mg·g- 1,respectively（ n = 3）. Conclusion： The method is simple,accurate and reproducible. It can be used to quantitatively analyze militarine,protocatechuic acid and caffeic acid in Bletilla Striata.

关 键 词：白及 1 4-二[4-(葡萄糖氧)苄基]-2-异丁基苹果酸酯 原儿茶酸 咖啡酸 液相串联质谱法 

分 类 号：R927.2[医药卫生—药学]