江苏省一起霍乱暴发疫情分离株耐药性及病原学特征分析  

Antibiotic resistance and molecular characterization of Vibrio cholera strains isolated from an outbreak of cholera epidemic in Jiangsu province

作　　者：董晨[1] 张雪峰[1] 鲍倡俊[1] 朱叶飞[1] 庄菱[1] 谈忠鸣[1] 钱慧敏[1] 汤奋扬[1] 

机构地区：[1]江苏省疾病预防控制中心急性传染病防制所卫生部肠道病原微生物重点实验室,南京210009

出　　处：《中华预防医学杂志》2015年第2期128-131,共4页Chinese Journal of Preventive Medicine

基　　金：江苏省科教兴卫工程（ZX201109、RC2011085）;江苏省预防医学科研项目（Y2012077）.志谢PulseNet China中心实验室、安徽省疾病预防控制中心提供的安徽蒙城霍乱暴发疫情的PFGE图谱,PulseNet China中心实验室在本文写作过程中的悉心指导

摘　　要：目的了解江苏省一起霍乱暴发疫情分离株的耐药性及病原学特征。方法对2010年9月江苏省淮安市某中学发生的一起霍乱暴发疫情所分离的4株霍乱弧菌分离株进行药物敏感性实验、生物分型实验、9种毒力相关基因检测、ctxB基因序列分析及PFGE分子分型分析。结果分离的4株霍乱弧菌对磺胺甲嗯唑／甲氧苄啶、红霉素、链霉素耐药，对阿米卡星、诺氟沙星、环丙沙星、庆大霉素、氯霉素、氨苄青霉素敏感；生物分型实验结果显示，4株菌均为埃尔托型霍乱弧菌；均携带所测9种毒力相关基因，即ctxA、ace、zot、toxR、tcpI、ompU、rtxC、tcpA、htyA基因，且CTXB氨基酸序列为古典型；4株菌经NotI酶切后的PFGE图谱为同一型别；经SfiI酶切后的PFGE图谱分为2个型别。结论此次暴发疫情的病原为产毒的非典型埃尔托霍乱弧菌流行株，且来自于共同的传染源，药物敏感实验可指导临床用药，以减少耐药株的产生。Objective To assess the antibiotic resistance and molecular characterization of cholera strains and to provide basis for clinical treatment and prevention of cholera. Methods 4 stains isolated from an outbreak of cholera epidemic in Huai＇an City in Jiangsu province in September 2010 were characterized using antibiotic susceptibility, biotype analysis, virluence genes detection, ctxB gene sequencing, and PFGE analysis. Results The 4 strains were all resistant to sulphamethoxazole/trimethoprim, erythromyein, streptomycin. High drug susceptibility of the samples was found to 6 kinds of antibiotics such as amikaein, norfloxacin, ciprofloxacin, gentamiein, chloramphenicol, ampieillin. The isolates expressed phenotypic traits of both serogroup O1 ogawa and E1 Tor and carried 9 kinds of virulence genes, ctxA, ace, zot, toxR, tcpI, ompU, rtxC, tcpA, and hlyA gene. They were also identified as harboring the classical ctxB genotype based on amino acid residue substitutions. The PFGE profiles of Not I showed a single banding pattern, while Sfi I＇s was 2 banding patterns. Conclusion The bacterium type of Vibrio cholerae causing the epidemic outbreak of cholera belonged to the atypical EL Tor variant which was also identified as toxieogenie strain. The mapping of the strains prompted that there should be the common contamination source. Drug sensitivity test can guide the clinical drug use, in order to reduce the emergence of resistant strains.

关 键 词：弧菌 霍乱 O1 基因 细菌 电泳 凝胶 脉冲场 

分 类 号：R516.5[医药卫生—内科学]