大鼠骨髓间充质干细胞的分离及分化潜能鉴定  

Isolation and identification of the differentiation potential of rat bone marrow-derived mesenchymal stem cells

作　　者：何琳琳 兰飞[2] 王永吉 

机构地区：[1]陕西理工学院维生素D生理与应用研究所,陕西汉中723000 [2]陕西理工学院数学与计算机科学学院,陕西汉中723000

出　　处：《陕西理工学院学报（自然科学版）》2015年第1期49-52,共4页Journal of Shananxi University of Technology:Natural Science Edition

基　　金：陕西省科技厅科技创新团队项目(2012KCT-29);陕西理工学院校级人才启动项目(SLGQD13(2)-26)

摘　　要：用全骨髓贴壁法从SD大鼠骨髓中分离骨髓间充质干细胞(bone marrow-derived mesenchymal stem cells,BM-MSCs)。为鉴定其成骨分化潜能,将大鼠BM-MSCs进行成骨诱导培养,第7天用碱性磷酸酶染色显示有碱性磷酸酶阳性细胞,第21天进行茜素红染色有矿化骨节形成。为鉴定其成脂分化潜能,将大鼠BM-MSCs先在成脂诱导培养基中培养,再换为在成脂肪维持培养基中培养,第10天进行油红O染色有脂滴形成。表明从SD大鼠骨髓中成功分离得到具有多向分化潜能的大鼠BM-MSCs,为其作为种子细胞应用于临床或研究奠定基础。In this study, bone marrow-derived mesenehymal stem cells （BM-MSCs） were isolated from Sprague-Dawley （SD） rat by using the whole bone marrow adherence method. To identify their osteogenie differentiation potential, BM-MSCs were cultured in osteogenie medium. Alkaline phosphatase staining was per-formed on the 7th day and there are alkaline phosphatase positive cells, and Alizarin Red S （ARS） staining was performed on the 21st day and bone nodules formation showed up. To identify their adipogenic differentiation potential, BM-MSCs were cultured in adipogenic induction medium, then switched in adipogenic maintenance medium, and on the 10th day oil red O staining showed lipid drop tormation. It indicates that rat BM- MSCs with multiple differentiation potential have been successfully isolated from SD rat bone marrow, which lays a foundation for the clinic and research using rat BM-MSCs as seed cells.

关 键 词：骨髓间充质干细胞 分离 分化潜能 

分 类 号：Q78[生物学—分子生物学]