反相流动注射抑制化学发光法测定头孢唑啉钠  

Reversed Flow Injection Inhibition Chemiluminescence System for the Determination of Sodium Cefazolin

作　　者：瞿鹏[1,2] 瞿昊宏 宋凌霄[3] 田爱华[3] 

机构地区：[1]商丘师范学院化学化工学院,纳米生物分析化学河南省高校重点实验室培育基地,河南商丘476000 [2]商丘市第一高级中学,河南商丘476000 [3]郑州大学化学与分子工程学院,河南郑州450052

出　　处：《分析科学学报》2015年第1期103-106,共4页Journal of Analytical Science

基　　金：国家自然科学基金(No.21475085;21175091;21105063)

摘　　要：基于头孢唑啉钠能抑制磷钼钒杂多酸氧化鲁米诺产生化学发光,结合流动注射分析技术,提出了测定头孢唑啉钠的化学发光新体系。头孢唑啉钠在3.0×10-7~8.0×10-5 g/mL质量浓度范围内与化学发光信号呈线性关系;检出限为8.0×10-8 g/mL;对5.0×10-6 g/mL的头孢唑啉钠进行11次连续测定,相对标准偏差为3.8%。该方法快速、灵敏,已经被成功地应用于头孢唑啉钠针剂的测定。Based on the inhibition effect of sodium cefazolin on the chemiluminescence（CL）reaction of luminol-vanadomolybdophosphoric heteropoly acid-NaOH system,a CL system for the determination of sodium cefazolin using reversed flow injection inhibition analysis is proposed in this paper.The linear range of calibration curve is 3.0×10-7-8.0×10-5 g/mL and the detection limit is 8.0×10-8 g/mL.The relative standard deviation is 3.8%（n=11）for 5.0×10-6 g/mL sodium cefazolin.The proposed CL system is desirable and has been applied to the determination of sodium cefazolin in its injections.

关 键 词：反相流动注射 化学发光 头孢唑啉钠 磷钼钒杂多酸 鲁米诺 

分 类 号：O657.39[理学—分析化学]