抗肿瘤活性化合物1403C的分离纯化  

Separation and Purification of Antitumor Bioactive Compound 1403C

作　　者：那可[1] 刘永双[1] 杨筱静[1] 赵文杰[1] 

机构地区：[1]中国医药工业研究总院上海医药工业研究院,创新药物与制药工艺国家重点实验室,上海200040

出　　处：《中国医药工业杂志》2015年第2期142-144,179,共4页Chinese Journal of Pharmaceuticals

基　　金：国家"海洋863"计划(2011AA090702)

摘　　要：从海洋红树内生真菌Fusarium proliferatum NO.1403的发酵液中分离纯化抗肿瘤活性产物1403C。发酵液经过滤得到菌丝体,用75%乙醇于70℃、p H 2.0条件下抽提3次,抽提上清液加水稀释至乙醇含量30%。经Amberchrom CG161树脂柱吸附去除弱极性杂质得到纯化液,纯化液浓缩近干后用80℃的75%乙醇结晶,得到红色晶体1403C。采用上述方法共制备6批样品,平均收率35.9%。An antitumor bioactive compound 1403C was separated and purified from fermentation broth produced by mangrove endophytic fungus Fusariurn proliferaturn. The mycelium was collected by filtration of fermentation broth and extracted three times with 75 % ethanol at 70 ℃ and pH 2.0. After that, the extract was diluted to ethanol concentration of 30 % by water followed by purification using Amberchrom CG161 resin column which could adsorb low-polar impurities. The refined solution was concentrated to anhydrous then crystallized with 75 % ethanol at 80 ℃ to obtain red crystalloid. Six batches of 1403C preparation were performed with an average yield of 35.9 %.

关 键 词：抗肿瘤活性化合物 1403C FUSARIUM proliferatum 分离纯化 吸附 结晶 

分 类 号：Q939.9[生物学—微生物学]