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作 者:叶春秀[1] 李全胜[1] 李有忠[1] 庄振刚[1] 谢宗铭[1]
机构地区:[1]新疆农垦科学院分子农业技术育种中心/作物种质创新与基因资源利用兵团重点实验室,新疆石河子832000
出 处:《西北农业学报》2015年第2期73-78,共6页Acta Agriculturae Boreali-occidentalis Sinica
基 金:国家自然科学基金(31460364);新疆农垦科学院"院科技引导计划项目"(41YYD201201);兵团生物种质资源专项(2012BD046)
摘 要:采用SSR分子标记对41份北疆地区早熟陆地棉品种进行遗传多样性分析,并构建其DNA指纹图谱。最终100对引物筛选出8对多态性较好引物,共检测50条带,其中多态性33条,多态性比率66%,每对引物平均扩增到6.25条条带。多态信息含量(PIC)为0.568 0~0.784 6,平均值为0.646 2。聚类分析表明,供试材料在遗传距离为0.42时可划分为3类,第3大类又可以划分为2大亚类,多数品种属于第3大类。利用其中7个特异性标记引物获得的数据构建供试材料的分子指纹图谱。新陆早系列在选育过程中亲本的来源较为广泛,遗传背景较复杂,获得的SSR分子指纹图谱能够用于新疆早熟陆地棉品种及相关材料的鉴定。In order to study the genetic diversity and phylogenetic relationship of earlier cultural of up land co earlier ttons in northern Xinjiang cultural upland cottons and to constru in Xinjiang and their DNA fingerprint. The genetic diversity of 41 ngerprints were established by Simple Sequence Re- peats . There were 50 bands were amplified, and 33 were polymorphisms. The percentage of polymor- phic loci was 66~. The genetic similarity coefficient of cuhivars was 0. 568 0--0. 784 6, and the aver- age value was 0. 646 2. Based on the unweighted pair group method with arithmetic average(UPG- MA), 41 materials were divided into 3 groups at the similarity coefficient of 0.42. Most of materials belong to the third group and the it could be divided into 2 small groups. The fingerprints were con- structed by 7 polymorphic SSR primers. The relationship of early maturing upland cotton in northern Xinjiang was complex, and the genetic diversity was abundant. SSR molecular marker could be used in genetic diversity and fingerprint analysis for it.
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