紫茎泽兰叶绿体基因RNA编辑位点的鉴定及分析  

Identification and Analysis of RNA Editing Sites in the Chloroplast Transcripts of Ageratina adenophora

作　　者：吕萌荔 王梦醒[1] 贾晓鸥[1] 邓平川[1] 聂小军[1] 宋卫宁[1] 

机构地区：[1]西北农林科技大学农学院,陕西杨凌712100

出　　处：《西北农业学报》2015年第2期127-133,共7页Acta Agriculturae Boreali-occidentalis Sinica

基　　金："973计划"重要外来物种入侵的生态影响机制与监控基础(2009CB119201);植物病虫害生物学国家重点实验室开放课题(SKLOF201314);国家自然科学基金(31471825)

摘　　要：RNA编辑是陆生植物叶绿体转录后基因表达调控的一种重要方式。本试验以紫茎泽兰为材料,利用生物信息学预测结合分子克隆及测序方法对其叶绿体的RNA编辑位点进行预测和分析。结果共预测到分布于19个基因的42个编辑位点,所有位点均为C到U的转换。编辑位点的发生位置分析发现,8个位于密码子第1位,34个位于密码子第2位,而密码子第3位未发现RNA编辑事件。同时,利用RT-PCR方法对其中4个基因的编辑位点进行验证,鉴定发现10个真实发生的编辑位点。进一步分析这10个位点发生编辑后对其编码蛋白质跨膜结构域和二级结构的影响,结果表明,ndhB-467的编辑会引起蛋白质跨膜结构的增加,ndhB-149的编辑会引起蛋白质二级结构的改变。RNA editing is an important approach to regulate the gene expression at post-transcription- al levels in chloroplast transcript in terrestrial plants. This paper predicted and analyzed the RNA edi- ting sites in the cp genome of A. adenophora using the Prep-Cp and CURE tools with molecular clo- ning and sequencing methods. Forty-two editing sites were identified and distributed in 19 protein- coding genes, of which all were C to U conversion. Among them, 8 RNA editing sites occurred in the first position of codons, and the rest 34 sites occurred in the second position of codons. No RNA edi- ting sites were detected in the third position of codon. Four genes and ten editing sites actual existed. Finally, the second structures and the transmembrane domains of these proteins were also analyzed using bioinformatics methods to identify the effect of the editing. Results indicated thatndhB-467 edi- ting can cause an increase in transmembrane protein domains while editing inndhB-149 will change the secondary structure.

关 键 词：紫茎泽兰 叶绿体 RNA编辑 鉴定分析 

分 类 号：S451[农业科学—植物保护]