补肾益髓法对辐射损伤小鼠造血生长因子的影响  

Effect of method of tonifying kidney and benefiting marrow on hemopoietic growth factors in mice exposed to ^(60)Co-γ rays

作　　者：王文娟[1] 方素萍[2] 刘咏梅[2] 吴志奎[2] 刘莉[2] 邹阳 李俊丽[2] 吕鑫霞[2] 阚杰[2] 

机构地区：[1]首都医科大学中医药学院北京100069 [2]中国中医科学院广安门医院

出　　处：《北京中医药大学学报》2015年第1期29-32,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)资助项目(No.2010CB530406);国家自然科学基金资助项目(No.81173167);首都医科大学科研基金资助项目(No.2012ZR19、2015ZR27)

摘　　要：目的探讨补肾益髓法的代表药益髓生血颗粒促进骨髓造血的机制。方法 78只雄性昆明种小鼠随机分为空白组、模型组、阳性药组、益髓生血颗粒高、中、低剂量组,每组13只。采用60Co-γ射线全身一次性照射造成小鼠骨髓急性损伤模型。以益髓生血颗粒高、中、低3个剂量及重组人粒细胞刺激因子分别作用于骨髓急性损伤小鼠14 d后,采用放射免疫法检测外周血及骨髓中干细胞因子(SCF)、白细胞介素-3(IL-3)、粒-单系祖细胞集落刺激因子(GM-CSF)、粒系祖细胞集落刺激因子(G-CSF)的含量。结果与模型组相比,益髓生血颗粒高剂量组血中GM-CSF、骨髓中IL-3和SCF含量明显升高(P<0.05),中剂量组血中GM-CSF、骨髓中IL-3含量明显升高(P<0.05),低剂量组血及骨髓中GM-CSF、骨髓中IL-3和SCF含量明显升高(P<0.05)。结论补肾益髓法可增加辐射损伤小鼠骨髓微环境中造血生长因子GM-CSF、IL-3、SCF的含量,从而有效促进髓系造血。Objective To explore the mechanism of Yisui Shengxue granule formulated by TCM method of tonifying kidney and benefiting marrow on promoting hemopoietic growth factors in bone marrows. Methods Altogether 78 male Kunming mice were randomly divided into six groups （n = 13 each ） ： blank group, model group, positive drug group, low dose, middle dose and high dose Yisui Shengxue granule group（ low-dose group, mid-dose group and high-dose group）. Except for blank group, mice in other five groups were whole-body exposed to 6~Co-~/ray once to establish the acute marrow injury model. Low-dose group, mid-dose group and high dose group were administrated intragastrically 3, 6, 12 g/kg Yisui Shengxue granule, while positive drug group was hypodermically injected recombinant human granulocyte colony-stimulating factor for 14 consecutive days. Stem cell factor （SCF） , interleukin-3 （IL- 3 ）, granulocyte-macrophage colony stimulating factor （GM-CSF）, and granulocyte colony stimulating factor （G-CSF） from blood serum and marrow liquid supernatant were detected by radioimmunoassay. Results Compared with model group, the contents of GM-CSF in blood, IL-3 in bone marrow increased significantly in all Chinese medicinal groups （P 〈 0. 05 ） ; furthermore, the contents of SCF increased in high-dose group and in low dose group （ P 〈 0.05 ）. Conclusion Method of Tonifying kidney and benefiting marrow improved the contents of GM-CSF, IL-3 and SCF of mice with acute marrow injure induced by exposure to 60Co-γ rays, leading to promoting the proliferation of myeloid hematopoietic cells.

关 键 词：补肾益髓法 益髓生血颗粒 辐射损伤 造血生长因子 小鼠 

分 类 号：R285.5[医药卫生—中药学]