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作 者:武尉杰[1] 万萌萌[1] 曹雨虹[1] 谭睿[1] 宋良科[1]
机构地区:[1]西南交通大学生命科学与工程学院,四川成都610031
出 处:《中华中医药学刊》2015年第2期313-315,I0007,I0008,共5页Chinese Archives of Traditional Chinese Medicine
基 金:国家药典委员会<中华人民共和国药典>2015版药典提高项目(201107009)
摘 要:目的:为准确鉴定川木通药材,更好地控制其药材质量提供一定的科学依据。方法:用石蜡切片对川木通的基源植物小木通Clematis armandii Franch.和绣球藤Clematis montana Buch.-Ham.的茎、叶、叶柄进行组织结构的比较鉴别,以TLC寻找可供定性鉴定的指标成分,用HPLC法筛选可供定量分析的含量测定方法。结果:川木通两种基源的茎(药材)性状相似,但维管束可区别两者,尤其叶柄的结构完全不同。6个采自不同地方的川木通药材样品在薄层色谱中均明显检出β-谷甾醇的特征性斑点;HPLC测定小木通和绣球藤茎(药材)中的β-谷甾醇含量的方法稳定,且精密度较高、重复性好,其β-谷甾醇的质量浓度分别在189.18-190.27和336.90-338.34μg·g^-1范围内。结论:TLC以三氯甲烷-乙酸乙酯(10∶1)为展开剂,以10%硫酸乙醇溶液显色,置紫外灯(365nm)下检视,斑点分离效果最佳,可作为川木通药材中β-谷甾醇的薄层专属性鉴别条件。HPLC拟定按干燥品计算,川木通药材中β-谷甾醇的含量不得少于0.018%。Objective: To provide definite science bases for its accurate identification and quality control. Methods:Comparative identification on the stem,petiole and leaf of the two original plants of Clematidis Armandii Caulis was carried out by the classical paraffin technique. We explored the index component for identification of Clematidis Armandii Caulis by TLC and established the quantitative analysis method by HPLC. Results: The rattan stems of Clematidis Armandii Caulis,originated from Clematis armandii Franch. and Clematis montana Buch.- Ham. were similar. However,they can be differentiated from each other by the structure of vascular bundle. Moreover,the structure of petiole was entirely different. The characteristic spots of beta- sitosterol in Clematidis Armandii Caulis from six different places were detected by TLC. HPLC was highly accurate and reproductive. The mass concentration of beta- sitosterol in Clematis armandii Franch. was 189. 18 - 190. 27 μg · g- 1and in Clematis montana Buch.- Ham. was 336. 90 - 338. 34 μg · g^- 1.Conclusion: The spots were apparent and effective separated when we used trichloromethane- ethyl acetate( 10 ∶ 1) for development and 10% vitriol ethanol to color under the UV light( 365 nm). This could be the TLC identification condition of beta- sitosterol in Clematidis Armandii Caulis. Calculated on dry product by HPLC,the beta- sitosterol in Clematidis Armandii Caulis could not be less than 0. 018%.
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