Bootstrap方法对参考测量程序测定肌酸激酶催化活性浓度不确定度的评价  

Uncertainty evaluation of catalytic activity of creatine kinase determined by reference measurement procedure using the Bootstrap method

作　　者：许丽丽[1] 王惠民[1,2] 王建新[1] 季伙燕[1] 景蓉蓉[1] 孟舒婷 

机构地区：[1]南通大学附属医院检验科,江苏南通226001 [2]南通大学检验医学研究所,江苏南通226001

出　　处：《临床检验杂志》2015年第1期66-69,共4页Chinese Journal of Clinical Laboratory Science

摘　　要：目的探讨用Bootstrap方法对参考测量程序测定肌酸激酶(CK)催化活性浓度的不确定度进行评价。方法用参考测量程序测量CK催化活性浓度;用Bootstrap方法对数据进行有放回的重抽样并进行分析,计算均数、不确定度;与GUM法评估结果进行比较。结果 Bootstrap方法评估时,标本A CK催化活性浓度的测量结果为(108.57±3.26)U/L(k=2),包含区间为[105.31 U/L,111.83 U/L],标本B结果为(375.15±8.86)U/L(k=2),包含区间为[366.29U/L,384.01 U/L];GUM方法评估时,标本A CK催化活性浓度测量结果为(108.57±3.38)U/L(k=2),包含区间为[105.19 U/L,111.95 U/L],标本B结果为(375.16±8.92)U/L(k=2),包含区间为[366.24 U/L,384.08 U/L]。结论 Bootstrap重抽样方法评估结果与GUM法一致,该法操作简便,便于推广。Objective To investigate the feasibility using the Bootstrap method to evaluate the uncertainty of catalytic activity of crea- tine kinase （CK） determined by reference measurement procedure. Methods The reference measurement procedure was used to determine the catalytic activity of CK. The Bootstrap method was used to analyze the re-sampling and calculate its mean and uncertainty. Then, the results obtained from the Bootstrap and the GUM method were compared. Results When the uncertainty was evaluated by the Bootstrap method, the results of samples A and B were （ 108.57 ±3.26） U/L （k = 2） with （ 105.31, 111.83 ） of confidence in- terval （ CI）, and （375.15 ± 8.86） U/L （k = 2） with （366.29, 384.01 ） of CI, respectively. When the uncertainty was evaluated by the GUM method, the results of samples A and B were （ 108.57 ± 3.38） U/L （ k = 2） with （ 105.19, 111.95） of CI, and （375.16±8.92） U/L （k = 2） with （366.24, 384.08 ） of CI, rcspectivcly. Ccncasion The obtained results from the Bootstrap method are consistent with those from the GUM method. Moreover, the Bootstrap method is simple, accurate, and easy to be applied.

关 键 词：BOOTSTRAP法 测量不确定度表达指南 测量不确定度 肌酸激酶 

分 类 号：R446[医药卫生—诊断学]