支气管哮喘患儿ORMDL3基因单核苷酸多态性对其发病的影响  

Single nucleotide polymorphisms in OR31DL3 gene impact on childhood asthma

作　　者：王桂兰[1] 王冰洁[1] 陈德晖[2] 王文祥[3] 黄娟[1] 容嘉妍[1] 刘翔腾[1] 姚苗苗[1] 杨赛[1] 鲁衍强 

机构地区：[1]南方医科大学附属中山市博爱医院小儿呼吸内科,广东中山528400 [2]广州医科大学附属第一医院儿科 [3]南方医科大学附属中山市小榄人民医院儿科 [4]上海靶向分子生物研究所

出　　处：《中华实用儿科临床杂志》2015年第4期282-285,共4页Chinese Journal of Applied Clinical Pediatrics

摘　　要：目的 探讨广东地区支气管哮喘患儿ORMDL3基因单核苷酸多态性（SNP）对其发病的影响。方法 采用多中心、大样本、前瞻性病例对照研究的方法，选取598例广东籍儿童（哮喘组300例、对照组298例），采用单因素Logistic回归分析对ORMDL3基因在广东地区哮喘儿童中占据优势的rs12603332 CC基因型与年龄、性别、家族史、特应性体质、呼吸道病毒感染史、吸入致敏因素、食物致敏因素等危险因素进行相关性分析。并进一步采用多因素Logistic回归分析ORMDL3基因 SNP rs12603332各基因型及与之有显著相关性的危险因素之间的相关作用。结果 ORMDL3基因 SNP rs12603332 CC 基因型与哮喘家族史（P =0.007，OR =1.845，95% CI1.178～2.889）及呼吸道病毒感染史（P =0.007，OR =1.976，95% CI1.206～3.235）有明显相关性，与其他危险因素未见明显相关性。SNP rs12603332 CC基因型、CT基因型、TT基因型均与哮喘家族史存在显著正相关作用，OR 分别为 22.644（95% CI9.390～54.606）、33.500（95% CI7.839～143.157）、11.167（95% CI1.319～94.559）。SNP rs12603332 CC基因型、CT基因型、TT基因型均与呼吸道病毒感染史存在显著正相关作用，OR分别为32.476（95% CI9.875～106.805）、12.667（95% CI3.699～43.375）、7.917（95% CI0.909～68.945）。结论 在有哮喘家族史的哮喘患儿中，ORMDL3基因SNP rs12603332 CC 基因型出现频率更高；SNP rs12603332的 CC 基因型的哮喘患儿可能更容易受到呼吸道病毒的侵袭进而导致哮喘；与其他哮喘危险因素相比，SNP rs12603332与哮喘家族史及呼吸道病毒感染史更易共同作用，导致广东地区儿童哮喘的发生。Objective To examine the single nucleotide polymorphisms in ORMDL3 gene impact on childhood asthma in Guangdong Chinese population. Methods A muhicenter, large sample, prospective case - control study was conducted. A total of 598 cases of Guangdong cadastral children （ 300 cases in asthmatic group and 298 cases in control group） were selected. Using univariate Logistic regression analysis was adopted to analyze the association between ORMDL3 gene SNP rs12603332 CC and the risk factors related to age, gender, family history, atopic history of physique, respiratory virus infection, suction sensitization, and food sensitization. Muhifactor Logistic regression analysis was selected to analyze the interactive effect between ORMDL3 gene and the risk factors for childhood asthma. Results ORMDL3 gene SNP rs12603332 CC genotype was significantly associated with family history（ P = 0. 007, OR = 1. 845, 95 % CI 1. 178 - 2. 889） and respiratory virus infection （ P = 0. 007, OR = 1. 976,95 % CI 1. 206 - 3. 235 ）. ORMDL3 gene SNP rs12603332 CC genotype, CT genotype and TT genotype had interactive effect with family history [ OR = 22. 644 （95 % CI 9. 390 -54. 606 ）, OR = 33. 500 （95 % CI 7. 839 - 143. 157 ）, and OR = 11. 167 （95 % CI 1.319 - 94. 559） ]. ORMDL3 gene SNP rs12603332 CC genotype, CT genotype and TT genotype had interactive effect with respiratory virus infection [ OR = 32. 476 （ 95 % CI 9. 875 - 106. 805 ）, OR = 12. 667 （ 95 % CI 3. 699 - 43. 375 ）, and OR = 7. 917 （95% CI 0. 909- 68. 945 ）]. Conclusions ORMDL3 gene SNP rs12603332 CC genotype indicates a high frequency in asthmatic children. The children who have ORMDL3 gene SNP rs12603332 CC genotype are easily attacked by respiratory virus. Compared with other asthma risk factors, SNP rs12603332 and family history of asthma or SNP rs12603332 and respiratory virus infection are more likely to have childhood asthma in Guangdong population.

关 键 词：ORMDL3基因 多态性 儿童哮喘 危险因素 相关作用 

分 类 号：R725.6[医药卫生—儿科]