两株具促生作用的苜蓿内生菌的分离纯化与鉴定  

Isolation and identification of two plant-growth promoting endophytes from alfalfa

作　　者：康慧颖 王伟[1] 刘佳莉[1] 程园园[1] 郭长虹[1] 郭东林[1] 

机构地区：[1]黑龙江省分子细胞遗传与遗传育种重点实验室哈尔滨师范大学生命科学与技术科学学院,黑龙江哈尔滨150025

出　　处：《微生物学通报》2015年第2期280-288,共9页Microbiology China

基　　金：国家自然科学基金项目(No.31170479;31470571);黑龙江省科技计划项目(No.GC12B304);黑龙江省研究生创新科研资金项目(No.YJSCX2012-191HLJ);哈尔滨师范大学遗传学大学生实践创新团队

摘　　要：【目的】分离具有植物促生功能的苜蓿内生菌。【方法】从苜蓿的新鲜组织中分离纯化菌株,进行16S r RNA基因序列分析,生理生化鉴定并检测内生菌对苜蓿的促生特性。【结果】获得两株苜蓿内生菌,成团泛菌ASR16和短小芽孢杆菌ALR33。两株菌株均能产生植物生长激素IAA和嗜铁素,能溶解磷,对苜蓿的促生作用明显。【结论】获得两株具有植物促生特性的苜蓿内生菌ASR16和ALR33,可作为微生物菌肥的研究基础。[Objective] To obtain endophytes which has the plant-growth promote function from alfalfa. [Methods] Two strains were isolated and purified from fresh tissues of alfalfa, and then analyzed by 16S rRNA gene sequence, identified by the physiological and biochemical methods. The plant-growth promote properties to alfalfa of two strains were detected. [Results] Two alfalfa endophytes, ASR16 （Pantoea vagans） and ALR33 （Bacillus pumilus） were obtained. Both of the endophytes can produce IAA and siderophore, have phosphate solubilization activity, and can promote the growth of alfalfa effectively. [Conclusion] Two plant endophytes, ASR16 and ALR33, with plant-growth promotion to alfalfa could be used as the basis of bacterial manure research.

关 键 词：苜蓿 内生菌 促生 

分 类 号：Q93[生物学—微生物学]