利用高效液相色谱法建立玉屏风总苷的指纹图谱  

Establishment of fingerprint of total glucosides of Yupingfeng by HPLC

作　　者：李德林[1] 许亮[1] 高建[2] 李留成[3] 赵萍[1] 张国庆[3] 黄赵刚[2] 牛海军[4] 李晓亮[4] 邵旭[4] 齐炼文[5] 

机构地区：[1]安徽中医药大学药学院,安徽合肥230038 [2]安徽医科大学第一附属医院药学部,国家中医药管理局中药化学三级实验室TCM-2009-202,安徽合肥230022 [3]安徽医科大学药学院,安徽合肥230032 [4]合肥七星医药科技有限责任公司,安徽合肥230088 [5]中国药科大学天然药物活性组分与药效国家重点实验室,江苏南京210009

出　　处：《中国医院药学杂志》2015年第3期205-209,共5页Chinese Journal of Hospital Pharmacy

基　　金：国家自然科学基金面上项目(编号:81274172);安徽省卫生厅中医药科研项目(编号:2012zy53);"天然药物活性组分与药效"国家重点实验室(中国药科大学)开放课题(编号:SKLNMKF201206);安徽省高等教育振兴计划人才项目(2013);2013年国家级大学生创新训练计划项目(编号:201310366029)

摘　　要：目的：以黄芪皂苷Ⅱ为参照峰,建立玉屏风总苷HPLC-ELSD的指纹图谱,对玉屏风总苷进行质量控制。方法：采用HPLC-ELSD法,色谱柱为Agilent C18（250 mm×4.6 mm,5μm）;以乙腈和0.1%甲酸为流动相进行梯度洗脱,柱温40℃,流速1.0ml·min^-1,漂移管温度110℃,N2载气流速3.30 L·min^-1。结果：建立了玉屏风总苷的HPLC-ELSD的指纹图谱,确定了17个共有峰,并鉴定了其中4个共有峰,各峰相对保留时间的RSD在0.1%-0.75%之间,相对峰面积的RSD在1.68%-4.82%之间。10批总苷样品的相似度均大于0.94。结论：该法简单、精密度、稳定性、重现性好,适用于玉屏风总苷的指纹图谱检测。OBJECTIVE To establish fingerprint of total glucosides of Yupingfeng by AstragalosideⅡ as the reference peak,and control the quality of total glucosides of Yupingfeng.METHODS HPLC-ELSD was used on an Aglient column（4.6 mm×250 mm,5μm）,with gradient mobile phase composed of acetonitrile and 0.1%formic acid.The column temperature was set at40 ℃ and flow rate of mobile phase 1.0mL·min^-1.The tube temperature of detector was 110 ℃,and flow rate of N2 was 3.30L·min^-1.RESULTS HPLC-ELSD fingerprint of the total glucosides of Yupingfeng was established,which determined 17 common peaks from10 batches of the total glucosides of Yupingfeng and indentified 4 of them.RSD values of relative retention times for peaks and peak areas were respectively in the range of 0.1%-0.75% and 1.68%-4.82%.The similarity of 10 batches of total glucosides was all greater than 0.94.CONCLUSION The established HPLC-ELSD fingerprint is simple and has desirable precision,stability,reproducibility,and it can be applied to determine the fingerprints of total glucosides of Yupingfeng.

关 键 词：玉屏风总苷 HPLC-ELSD 指纹图谱 

分 类 号：R972[医药卫生—药品]