不同纯度的有机溶剂对2株肿瘤细胞的抑制作用  

Inhibitory effect of different purity organic solvents on two tumor cells

作　　者：周松林[1] 林映莹[1] 赵焕阁[1] 黄用豪[1] 

机构地区：[1]海南医学院热带病重点实验室,海口571199

出　　处：《中国当代医药》2015年第5期144-145,148,共3页China Modern Medicine

基　　金：海南省自然科学基金(808157)

摘　　要：目的给脂溶性的抗肿瘤药物筛选高溶解性、低细胞毒性的有机溶剂。方法采用MTT法,按照不同纯度,对8种能溶于水的有机溶剂,测试其对黑色素瘤B16、人胃癌细胞SGC-7901两种细胞株的细胞毒性。结果不同有机溶剂对不同细胞株的抑制皆不同,甲醇与丙酮对两种细胞株的毒性相对都较弱,纯度越高的有机溶剂对细胞毒害越小。结论光谱纯的甲醇与丙酮是脂溶性抗肿瘤药物有效的有机溶剂。Objective To select organic solvents with low cytotoxicity for dissolve some compounds which had bad water-solubility in screening antitumor agent compound. Methods The cytotoxic effect of 8 organic solvents both can dissolve in water and fat soluble organic solvents with different purity on two tumor cell lines （B16 and SGC-7901）were tested by MTT. Results Different organic solvents inhibit different cell lines were different,the effect of methanol and acetone toxicity on two kinds of cell lines were relatively weak.The higher the purity of the organic solvent on two tu- mor cell had low toxicity. Conclusion The spectrum class pure methanol and acetone are effective organic solvent of fat-soluble anticancer drugs.

关 键 词：MTT 细胞毒性 有机溶剂 纯度 

分 类 号：R915[医药卫生—微生物与生化药学]