不同针刺刺激量对寒凝证类痛经大鼠子宫微循环及其相关物质的影响  

Effect of Different Acupuncture Stimuli on Uterine Micro-circulation and Circulationrelated Substances of Dysmenorrheal Rats with Cold Stagnation Syndrome

作　　者：章庆庆[1] 朱世鹏[2] 罗丽 郭孟玮[1] 张玲[1] 申松希[1] 齐丹丹[1] 杨佳敏[1] 沈小雨[1] 许咏思[1] 王洋[1] 赵雅芳[1] 嵇波[1] 任晓暄[1] 张露芬[1] 朱江[1] 李晓泓[1] 

机构地区：[1]北京中医药大学针灸推拿学院,北京100029 [2]南京中医药大学第二临床医学院,江苏南京210046

出　　处：《中国中医药信息杂志》2015年第3期51-55,共5页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(2012CB518506;2006CB504503;2005CB523308);教育部高等学校博士点科研基金(20090013110005);国家自然科学基金面上项目(30973793)

摘　　要：目的从子宫微循环角度探讨不同针刺刺激量对寒凝证类痛经大鼠的针刺效应。方法动情间期3月龄SD雌性大鼠32只,采用随机数字法分为盐水组、模型组、刺激量A组和刺激量B组,每组8只。采用全身冷冻结合苯甲酸雌二醇注射法造模,刺激量A组予粗针深刺加手法干预,刺激量B组予细针浅刺不施手法。观察各组子宫毛细血管、微血管管径及血浆血栓素B2(TXB2)、6-酮-前列腺素F1α(6-keto-PGF1α)含量。结果与盐水组比较,模型组5、10、20、30 min各时点毛细血管管径明显缩小(P<0.01),5、10、20、30、40 min各时点微血管管径明显缩小,血浆6-keto-PGF1α含量明显降低、TXB2/6-keto-PGF1α明显升高(P<0.01)。与模型组比较,刺激量A组5、10、20、30 min各时点毛细血管管径明显扩张(P<0.05),5、10、20、30、40 min各时点微血管管径明显扩张,血浆6-keto-PGF1α含量明显升高、TXB2/6-keto-PGF1α明显降低(P<0.05,P<0.01);刺激量B组在20、30 min微血管管径明显扩张(P<0.05),血浆TXB2/6-keto-PGF1α明显降低(P<0.05)。与刺激量B组比较,刺激量A组5、10、20、30 min各时点毛细血管管径明显扩张(P<0.05),5、10、20、30、40 min各时点微血管管径明显扩张(P<0.01)。结论寒凝证类痛经大鼠存在明显的子宫微循环障碍及循环相关物质的紊乱。A、B刺激量均能改善痛经时子宫微循环,其机制可能与恢复失衡的TXB2及6-keto-PGF1α相关。Objective To explore the effect of different acupuncture stimuli on uterine micro-circulation of dysmenorrheal rats with cold stagnation syndrome. Methods Totally 32 three-month old female SD rats in diestrus were randomly divided into saline control group, model group, A stimuli group, and B stimuli group, 8 rats in each group. Model group and treatment groups were given whole body freezing combined with estradiol benzoate injection method to establish models. A stimuli group was given deep puncture with manipulation, while B stimuli group was treated by shallow puncture without manipulation. Diameter of uterine capillary,micro-vessel, TXB2, and 6-keto-PGF1αlevels were observed in each group. Results Compared with the saline group, capillary diameter in model group was significantly reduced at 5, 10, 20, 30 min time point （P〈0.01）;micro-vascular diameter was significantly reduced at 5, 10, 20, 30, 40 min time point （P〈0.01）;plasma 6-keto-PGF1α levels decreased （P〈0.01）;TXB2/6-keto-PGF1αincreased significantly （P〈0.01）. Compared with model group, capillary diameter in A stimuli group enlarged at 5, 10, 20, 30 min time point （P〈0.05）, micro-vascular diameter dilated at 5, 10, 20, 30, 40 min time point （P 〈0.01）, plasma 6-keto-PGF1α level increased （P 〈0.05）, TXB2/6-keto-PGF1αdecreased significantly （P〈0.05）;micro-vascular diameter in B stimuli group dilated at 20, 30 min time point （P〈0.05）, plasma TXB2/6-keto-PGF1α decreased significantly （P〈0.05）. Compared with B stimuli group, capillary diameter in A stimuli group dilated at 5, 10, 20, 30 min time point （P〈0.05） and micro-vascular diameter dilated at 5, 10, 20, 30, 40 min time point significantly （P〈0.01）. Conclusion Dysmenorrheal rats with cold stagnation syndrome show obvious disorder of the uterus micro-circulation and circulation related substances. Both A and B acupuncture stimuli improved uterus micro-circulation of dysmenorrheal rats with cold stagnation syndrome, and its mecha

关 键 词：不同针刺刺激量 寒凝证类痛经大鼠 子宫微循环 血栓素B2 6-酮-前列腺素-F1α 

分 类 号：R245[医药卫生—针灸推拿学]