白藜芦醇对粥样硬化模型兔血脂及ADRP表达的影响  

Influence of resveratrol on blood lipid level and ADRP expression in atherosclerosis model rabbits

作　　者：张洪霞[1,2] 李文杰[2] 王丽明[1] 

机构地区：[1]沈阳市第七人民医院心血管科,辽宁沈阳110003 [2]辽宁中医药大学附属第一医院心内一科

出　　处：《心血管康复医学杂志》2015年第1期20-23,共4页Chinese Journal of Cardiovascular Rehabilitation Medicine

摘　　要：目的:探讨白藜芦醇对动脉粥样硬化(AS)模型兔血脂及脂肪分化相关蛋白(ADRP)表达的影响。方法:将新西兰兔36只随机分为4组(每组9只):正常对照组、AS模型组、白藜芦醇低剂量组和白藜芦醇高剂量组。治疗给药15周,检测各组治疗前后血清脂蛋白的表达水平,主动脉组织脂肪细胞中ADRP表达,兔主动脉组织脂肪细胞ADRP mRNA的表达,并进行统计学比较。结果:15周末与AS模型组相比,白藜芦醇低、高剂量组血清总胆固醇[TC,(18.25±4.35)mmol/L比(12.79±2.45)mmol/L、(10.35±2.80)mmol/L],甘油三酯[TG,(3.15±0.94)mmol/L比(2.63±0.75)mmol/L、(1.18±0.64)mmol/L]及低密度脂蛋白胆固醇[LDL-C,(15.18±3.65)mmol/L比(8.35±1.15)mmol/L、(7.18±1.75)mmol/L]含量明显下降,高密度脂蛋白胆固醇[HDL-C,(1.02±0.36)mmol/L比(3.22±1.02)mmol/L、(2.72±0.82)mmol/L]明显升高(P<0.05或P<0.01),白藜芦醇高剂量组甘油三酯[TG,(3.15±0.94)mmol/L比(1.18±0.64)mmol/L]水平显著降低(P<0.01);白藜芦醇低、高剂量组主动脉组织脂肪细胞中ADRP组化染色[(0.43±0.05)比(0.35±0.04)、(0.38±0.15)]及ADRPmRNA的表达[(2.47±0.94)比(1.30±0.49)、(1.83±0.61)]均明显减弱(P均<0.01)。结论:白藜芦醇能抑制动脉粥样硬化兔主动脉组织脂肪细胞中ADRP及其mRNA的过度表达,降低血脂,有利于延缓动脉粥样硬化进程。Objective：To explore the influence of resveratrol on blood lipid level and expression of adipose differenti-ation related protein （ADRP）in atherosclerosis （AS）model rabbits.Methods：A total of 36 New Zealand rabbits were randomly and equally divided into normal control group,AS model group,low dose resveratrol group （low dose group）and high dose resveratrol group （high dose group）.Rabbits received 15-week administration,expression level of serum lipoprotein,ADRP and ADRPmRNA expressions in adipocytes of rabbit aortic tissue were measured and compared among all groups before and after treatment.Results：Compared with AS model group at the end of 15th week,there were significant reductions in serum concentrations of total cholesterol [TC,（18.25±4.35）mmol/L vs.（12.79±2.45）mmol/L,（10.35±2.80）mmol/L],triglyceride [TG, （3.15±0.94）mmol/L vs. （2.63± 0.75）mmol/L、（1.18±0.64）mmol/L]and low density lipoprotein cholesterol [LDL-C,（15.18±3.65）mmol/L vs.（8.35±1.15）mmol/L,（7.18±1.75）mmol/L],and significant rise in level of high density lipoprotein choles-terol [HDL-C,（1.02±0.36）mmol/L vs.（3.22±1.02）mmol/L,（2.72±0.82）mmol/L]in low dose group and high dose group （P 〈0.05 or 〈0.01）,and significant reduction in level of triglyceride [TG,（3.15±0.94）mmol/L vs.（1.18±0.64）mmol/L]in high dose group,P 〈0.01;ADRP histochemical staining [（0.43±0.05）vs.（0.35± 0.04）,（0.38±0.15）]and ADRPmRNA expression [（2.47±0.94）vs.（1.30±0.49）,（1.83±0.61）]significantly weakened in adipocytes of aortic tissue in low dose group and high dose group,P 〈0.01 all. Conclusion：Resveratrol can inhibit the overexpression of ADRP and its mRNA in adipocytes of aortic tissue in AS rabbits,reduce blood lipid levels,and help to delay the AS progression.

关 键 词：动脉粥样硬化 高脂血症 藜芦属 

分 类 号：R543.509[医药卫生—心血管疾病]