β-谷甾醇对脂多糖诱导的小鼠急性肺损伤的保护作用研究  

Protective Effects of β-Sitosterol on Acute Lung Injury Induced by Lipopolysaccharide in Mice

作　　者：姚凤 周清燕 熊瑛[3] 关爽[3] 

机构地区：[1]湖南省湘潭市动物卫生监督所,湖南湘潭411104 [2]湖南省湘潭县畜牧兽医水产局,湖南湘潭411228 [3]吉林大学农学部,长春130062

出　　处：《中国农学通报》2015年第2期55-61,共7页Chinese Agricultural Science Bulletin

摘　　要：为了探究β-谷甾醇对LPS诱导的急性肺损伤小鼠的保护作用及其可能的机制,利用酶联免疫吸附法(ELISA)测定β-谷甾醇对RAW 264.7细胞产生肿瘤坏死因子(TNF-α)和白细胞介素-6(IL-6)的影响。给BALB/c小鼠鼻腔滴注LPS(0.5mg/kg)构建小鼠急性肺损伤模型,滴鼻24 h后检测各指标。ELISA检测小鼠肺泡灌洗液中(BALF)的炎性细胞因子含量;称重法检测肺组织湿/干重比和肺含水量;HE染色法观察肺组织病理形态学变化;应用蛋白印迹法(western blot)检测小鼠肺脏中核转录因子-κB(NF-κB)信号转导通路中相关蛋白的含量变化。结果表明,β-谷甾醇能剂量依赖性降低RAW 264.7细胞上清及ALI小鼠BALF中TNF-α和IL-6的表达水平。肺脏称重结果和病理形态学结果显示β-谷甾醇可明显减轻LPS诱导的肺水肿和炎症反应。另外,western blot结果表明β-谷甾醇不仅能够下调NF-κB p65的活化,还抑制了NF-κB阻断剂IκBα的磷酸化。β-谷甾醇对LPS所致的小鼠急性肺损伤有较好的保护作用,其保护机制可能与阻止炎症因子(TNF-α、IL-6)的释放和下调NF-κB信号转导通路的活化有关。The paper aims to investigate the mechanism and protective effects of β-sitosterol on mice with Lipopolysaccharide （LPS）-induced acute lung injury （ALI）. We evaluated the effect of β-sitosterol on LPS- induced production of TNF-α and interleukin （IL）-6 in the culture supernatants of RAW 264.7 cells by enzyme-linked immunosorbent assay （ELISA）. Model of ALI in BALB/c mice was built by LPS （0.5 mg/kg） intranasal instillation and the severity of pulmonary injury was evaluated 24 h after LPS challenge. The inflammatory cytokines were assayed with ELISA in bronchoalveolar lavage fluid （BALF）; the ratio of lung wet weight to dry weight （W/D） and water content were also measured; lung tissues were stained with Hematoxylineosin to observe the pathological. In addition, the activity changes of nuclear factor-KB （NF-KB） were detected by western blot. β-sitosterol could dose-dependently decrease the levels of TNF-α and IL-6 in vitro and in vivo. Pretreatment with β-sitosterol was found to decrease the W/D ratio and water content of the lung tissue and suppress lung edema and pulmonary alveolar damage at the same time. In addition,β-sitosterol regulated inflammatory cytokines （TNF-α, IL-6） and NF-KB expressions.

关 键 词：Β-谷甾醇 脂多糖 急性肺损伤 细胞因子 NF-ΚB 

分 类 号：S8[农业科学—畜牧兽医]