姜黄素3种单体的分离纯化  

Extraction and purification of three monomers in curcuminoids

作　　者：方颖[1] 谢渝[2] 卢皓瑶 连晓东[2] 林伟民[2] 刘欣[2] 高向阳[2] 

机构地区：[1]华南农业大学公共基础课实验教学中心,广东广州510642 [2]华南农业大学食品学院,广东广州510642

出　　处：《中国酿造》2015年第1期94-98,共5页China Brewing

基　　金：广东高校国际科技合作创新平台项目(2013gjhz0003);广东省国家级大学生创新创业训练计划项目(20121056412161)

摘　　要：以中药姜黄饮片为原料提取姜黄色素,采用乙醇超声波法提取、大孔树脂吸附分离以及硅胶柱色谱法过柱洗脱依次得到3种姜黄色素单体,并通过薄层层析和HPLC对姜黄素3种单体进行纯度检测。以DM301大孔树脂吸附纯化姜黄色素,提取率为58.09%;利用硅胶柱色谱分离法对姜黄色素提取液进一步纯化得到3种姜黄素单体:姜黄素(curcumin Ⅰ)、脱甲氧基姜黄素(demethoxycurcumin Ⅱ)和双脱甲氧基姜黄素(bisdemethoxycurcumin Ⅲ),薄层层析检测结果显示各单体斑点明显、均一,纯度较高。高效液相色谱法确定色谱条件:色谱柱为DiamonsilRC18(5μm,250 mm×4.6 mm),流动相为乙腈-0.4%冰醋酸体积比48:52,检测波长为254 nm,在该HPLC条件下,总姜黄素中的3种单体能够实现基线分离,分离度较好。Traditional Chinese medicine curcuminoids decoction pieces were extracted by ultrasonic extraction with ethanol as solvent,and purified by DM301 type macroreticular resins adsorption,and then three kinds of curcumin monomers were obtained.Three monomers were further separated by silica gel column chromatography,they were identified by thin-layer chromatography,and analyzed by HPLC.The conclusion was that the extraction rate was 58.09％ after purified by macroreticular resins DM301 adsorption.The three monomers were curcumin Ⅰ,demethoxycurcumin Ⅱ,bisdemethoxycurcumin Ⅲ.The thin-layer chromatography results showed that the monomer spots were clear,uniform with high purity.The HPLC condition was Diamonsil（R） C18 column（5 μm,250 mm×4.6 mm） with isocratic elution of the mobile phase consisted of 0.4％ acetonitrile and glacial acetic acid 48∶52 （V/V）,the detection wave length was 254 nm.The results showed the baseline separation of three monomers from reference substance could be realized under this HPLC condition.

关 键 词：姜黄素单体 大孔树脂 硅胶柱色谱法 高效液相色谱 

分 类 号：TS202.3[轻工技术与工程—食品科学]