早期胚胎停止发育绒毛中IGF2、GCN5和p300/CBP的表达  

Expressions of IGF2, GCN5 and p300/CBP in human villus of early embryonic death

作　　者：刘蓉[1] 宗实[1] 史娟娟[1] 王珺[1] 

机构地区：[1]中国医科大学附属盛京医院妇产科,辽宁沈阳110004

出　　处：《中国现代医学杂志》2015年第4期25-29,共5页China Journal of Modern Medicine

基　　金：国家自然科学基金(No:81100433);辽宁省科技厅计划项目(No:2010225031);辽宁省教育厅高等学校科学研究项目(No:L2010654)

摘　　要：目的研究胰岛素样生长因子2(IGF2)及组蛋白乙酰基转移酶(HATs)相关因子组蛋白乙酰基转移酶(GCN5)、p300/CREB结合蛋白(p300/CBP)在正常及早期胚胎停止发育患者绒毛中的表达及三者间的相关性。方法采用免疫组化SP法检测21例正常早期妊娠绒毛、29例胚胎停止发育绒毛组织中IGF2、GCN5和p300/CBP的表达情况。结果早期胚胎停止发育绒毛中,IGF2、GCN5和p300/CBP的阳性表达显著低于正常早孕绒毛(P=0.001、P=0.002和P=0.003);并且这些指标在胚胎停止发育绒毛中的表达,两两间均存在显著正相关(r=0.563、0.622和0.563,P<0.00)。结论 IGF2、GCN5及p300/CBP的表达下调可能与早期妊娠胚胎停止发育有关,GCN5及p300/CBP的表达下调有可能参与早期绒毛发育过程中IGF2的表观遗传调控。[ Objective ] To detect the expressions of insulin-llke growth factor 2 （IGF2）, histone acetyhransferases general control of nucleotidesynthesis（GCN5） and p300/CREB-binding protein（p300/CBP） in normal villus and villus of early embryoic death patients and to explore the relativity among the expressions of GCN5, p3OO/CBP and IGF2 in villus of early embryonic death. [Methods ] Immunohistochemical SP method was used to detect the expression levels of IGF2,GCN5 and p300/CBP in 21 normal early pregnancy villus samples （control group） and 29 early embryonic death villus samples. [Results] The positive expression rates of IGF2, GCN5 and p300/CBP were significantly higher in normal early pregnancy villus than those in early embryonic death villus （P =0.001, P =0.002, P =0.003）. Positive correlations were found between any two of them （r =0.563/0.622/0.563, P 〈0.00）. [ Conclusion ] The down-regulation of IGF2, GCN5 and p300/CBP may be related to the occurrence of early embryonic death. GCN5 and p300/CBP may take their parts in the epigenetic regulation of IGF2 in early villus development.

关 键 词：胚胎停止发育 绒毛 胰岛素样生长因子2 组蛋白乙酰转移酶 相关因子组蛋白乙酰基转移酶 p300/CREB-结合蛋白 免疫组织化学 

分 类 号：R714.2[医药卫生—妇产科学] R392.11[医药卫生—临床医学]