微酸环境响应的聚天冬氨酸修饰脂质体的制备与表征  

Preparation and characterization of subacid environment responsive polyaspartic acid modified liposomes

作　　者：王丽琳[1] 沈骧一 苏海佳[1] 曹辉[1] 

机构地区：[1]北京化工大学北京市生物加工过程重点实验室,北京100029

出　　处：《化工学报》2015年第3期1234-1239,共6页CIESC Journal

基　　金：国家重点基础研究发展计划项目(2014CB745100);国家高技术研究发展计划项目(2012AA021402);教育部留学回国人员科研启动基金资助项目(LXJJ2012-001)~~

摘　　要：利用带负电的聚天冬氨酸分子与部分带正电的磷脂双分子层之间的静电吸附作用,通过'一步法'成功制备了聚天冬氨酸(PASP)修饰脂质体(PLPs),实现了修饰脂质体微酸环境响应性。将脂质体制备与PASP修饰同时完成的'一步法'大大简化了制备工艺,提高了脂质体(PLPs)的制备效率。通过单因素筛选确定了p H敏感性显著的修饰脂质体制备条件,即外水相溶液PASP浓度2.5%(质量分数),p H8.5。透射电子显微镜照片显示PLPs由于修饰剂的存在具有更大的粒径,且表面电负性高,证明了'一步法'成功制备了p H敏感性修饰脂质体。PASP modified liposomes （PLPs） were prepared by using negatively charged PASP chains linked to the head of positively charged phospholipid via electrostatic adsorption. A ＂one-step＂ method was developed to form and modify the liposomes. Single factor experiments were conducted to prepare high pH sensitive PLPs. The optimal formula in preparing PLPs was 2.5%（mass） PASP solution with pHS.5. TEM results showed that PASP modified liposomes had a larger particle size and their surface with higher electro-negativity. PASP modified liposomes are a kind of relatively stable emulsion system.

关 键 词：PH敏感 聚天冬氨酸 脂质体 制备 纳米粒子 

分 类 号：R944.1[医药卫生—药剂学]